色谱

色谱 ›› 2010, Vol. 28 ›› Issue (08): 809-812.DOI: 10.3724/SP.J.1123.2010.00809

• 技术与应用 • 上一篇    下一篇

高速逆流色谱法分离纯化续随子种子中的七叶内酯

余霞1,2, 张卫明2, 石雪萍2, 孙力军2*   

  1. 1. 南京师范大学生命科学学院, 江苏 南京 210046; 2. 南京野生植物综合利用研究院, 江苏 南京 210042
  • 收稿日期:2010-04-16 修回日期:2010-06-23 出版日期:2010-08-28 发布日期:2010-08-28
  • 通讯作者: 孙力军,博士,主要研究方向为生物技术.
  • 基金资助:

    国家科技支撑计划项目(No. 2006BAD07A04)和江苏省自然科学基金项目(No. BK2008095).

Isolation and purification of esculetin from the seeds of Euphorbia lathyris L. using high-speed counter-current chromatography

YU Xia1,2, ZHANG Weiming2, SHI Xueping2, SUN Lijun2*   

  1. 1. College of Life Sciences, Nanjing Normal University, Nanjing 210046, China; 2. Institute for the Comprehensive Utilization of Wild Plants, Nanjing 210042, China
  • Received:2010-04-16 Revised:2010-06-23 Online:2010-08-28 Published:2010-08-28

PDF

444

被引次数

35

摘要： 建立了高速逆流色谱(HSCCC)技术分离纯化续随子种子中七叶内酯的方法。将续随子种子的乙酸乙酯萃取物直接进行高速逆流色谱分离,考察了不同溶剂系统的分离效果。结果表明,最佳的溶剂系统为氯仿-甲醇-水(体积比为4:3:2),以其上相为固定相,下相为流动相。从200 mg续随子种子乙酸乙酯萃取物中分离得到80 mg七叶内酯,纯度为99.04%。HSCCC技术可高效分离纯化续随子种子中的七叶内酯,为得到高纯度的七叶内酯提供了制备技术。

关键词: 高速逆流色谱, 七叶内酯, 续随子种子

Abstract: A method for the isolation and purification of esculetin from the seeds of Euphorbia lathyris L. was developed using high-speed counter-current chromatography (HSCCC). The ethyl acetate extract of the seeds of Euphorbia lathyris L. was separated by the HSCCC directly. Different solvent systems were investigated, and the results showed that the best solvent system was the two-phase solvent system composed of chloroform-methanol-water (4:3:2, v/v/v). The lower phase was used as the mobile phase and the upper phase was used as the stationary phase. A total of 80 mg esculetin with the purity of 99.04% was successfully obtained from 200 mg crude extract of the seeds of Euphorbia lathyris L. The results indicate that optimized HSCCC offers a preferred method for the preparation of esculetin from the seeds of Euphorbia lathyris L.

Key words: esculetin, seeds of Euphorbia lathyris L., high-speed counter-current chromatography (HSCCC)