色谱

色谱 ›› 2010, Vol. 28 ›› Issue (09): 905-907.DOI: 10.3724/SP.J.1123.2010.00905

• 技术与应用 • 上一篇    下一篇

高效液相色谱法测定鸡组织与鸡蛋中氯苯胍的残留量

刘勇军1*, 吴银良2, 姜艳彬1   

  1. 1. 中国动物疫病预防控制中心, 北京 100125; 2. 宁波市农业科学院, 浙江 宁波 315000
  • 收稿日期:2010-06-01 修回日期:2010-07-19 出版日期:2010-09-28 发布日期:2010-09-28
  • 通讯作者: 刘勇军,博士,工程师,主要研究方向为药理毒理与兽药残留研究.

Determination of robenidine residue in chicken tissues and eggs by high performance liquid chromatography

LIU Yongjun1*, WU Yinliang2, JIANG Yanbin1   

  1. 1. China Animal Disease Control Center, Beijing 100125, China; 2. Ningbo Academy of Agricultural Sciences, Ningbo 315000, China
  • Received:2010-06-01 Revised:2010-07-19 Online:2010-09-28 Published:2010-09-28

PDF

470

被引次数

24

摘要： 建立了测定鸡组织与鸡蛋中氯苯胍残留的高效液相色谱法。试样用乙腈提取,经HLB固相萃取柱净化和流动相定容后,采用高效液相色谱法测定。以乙腈-0.05 mol/L NH4H2PO4缓冲液(6:4, v/v, pH 6.5)为流动相,流速为1.0 mL/min,于317 nm波长下检测。氯苯胍在10～1000 μg/L范围内具有良好的线性关系;方法的检出限(信噪比为3)为10 μg/L,定量限(信噪比为10)为15 μg/kg;回收率为73.1%～88.7%。该法操作简便、快速、灵敏、准确,样品处理简便易行,适用于测定鸡组织与鸡蛋中残留的氯苯胍。

关键词: 高效液相色谱法, 鸡蛋, 鸡组织, 氯苯胍

Abstract: A method for the determination of robenidine residue in chicken tissues and eggs by high performance liquid chromatography (HPLC) was established. The samples were extracted with acetonitrile and purified by an HLB solid-phase extraction (SPE) cartridge. The extract was analyzed by HPLC with acetonitrile-0.05 mol/L NH4H2PO4 buffer (6:4, v/v, pH 6.5) as the mobile phase. The flow rate of the mobile phase was 1.0 mL/min and detection wavelength was 317 nm. There was a good linear correlation between the peak areas and the concentrations of robenidine in the range of 10~1000 μg/L. The limit of detection (S/N=3) was 10 μg/L, and the limit of quantification (S/N=10) was 15 μg/kg. The recoveries of robenidine were 73.1%~88.7% at the spiked levels of 15, 50 and 100 μg/kg. The results demonstrate that the method is easy, fast, sensitive, and suitable for the confirmation and quantification of robenidine residue in chicken tissues and eggs. The clean-up effect, sensitivity, accuracy and precision can all meet the analysis requirement.

Key words: chicken tissues, eggs, robenidine, high performance liquid chromatography (HPLC)