色谱

色谱 ›› 2010, Vol. 28 ›› Issue (09): 908-911.DOI: 10.3724/SP.J.1123.2010.00908

• 技术与应用 • 上一篇    

高效液相色谱-蒸发光散射检测法测定番茄中的番茄皂苷A

LU Fenglai, LIU Jinlei, CHEN Yueyuan, WU Jianzhang, LI Dianpeng*   

  1. 广西壮族自治区、中国科学院广西植物研究所, 广西 桂林 541006
  • 收稿日期:2010-06-28 修回日期:2010-08-11 出版日期:2010-09-28 发布日期:2010-09-28
  • 通讯作者: 李典鹏,博士,研究员,主要从事植物活性物研究与利用研究.
  • 基金资助:

    广西科学基金应用基础研究专项项目(No.桂科基0991020)和桂林市科学研究与技术开发项目(No.20100103-3).

Determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography with evaporative light scattering detection

卢凤来, 刘金磊, 陈月圆, 吴建璋, 李典鹏*   

  1. Guangxi Institute of Botany, Chinese Academy of Sciences, Guilin 541006, China
  • Received:2010-06-28 Revised:2010-08-11 Online:2010-09-28 Published:2010-09-28

PDF

397

被引次数

10

摘要： 建立了测定番茄原料中的番茄皂苷A含量的高效液相色谱-蒸发光散射检测法(HPLC-ELSD)。将实验条件优化后,运用该方法分离番茄皂苷A。结果表明,该方法在番茄皂苷A的质量为0.61～3.05 mg范围内具有良好的线性相关性(r=0.9995),平均回收率为97.9%～104.8%,相对标准偏差(RSD)≤4.14%(n=5)。该方法快速、准确,样品处理简单,可用于番茄原料及其提取物中番茄皂苷A的含量测定与质量控制。

关键词: 番茄, 番茄皂苷A, 高效液相色谱法, 蒸发光散射检测

Abstract: A method for the determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) was established. The results showed that esculeoside A had good linearity in the range of 0.61~3.05 mg with the correlation coefficient of 0.9995. The average recoveries were 97.9%~104.8% with the relative standard deviations (RSD) ≤4.14% (n=5). The method is simple, sensitive and suitable for the analysis of esculeoside A and quality control for the raw material and extract of Lycopersicon esculentum MILL.

Key words: esculeoside A, evaporative light scattering detection (ELSD), Lycopersicon esculentum MILL, high performance liquid chromatography (HPLC)