色谱

色谱 ›› 2010, Vol. 28 ›› Issue (10): 935-939.

• 研究论文 • 上一篇    下一篇

原料奶在HPLC-MS负离子模式下的指纹图谱研究

王颜红1,赵春杰2,张红1,王世成1,林桂凤1,曾庆芳3   

  1. 1. 中国科学院沈阳应用生态研究所
    2. 沈阳药科大学药学院
    3. 沈阳药科大学
  • 收稿日期:2010-07-12 修回日期:2010-09-06 出版日期:2010-10-28 发布日期:2010-10-28
  • 通讯作者: 王颜红

Research of Raw Milk Fingerprint Based on HPLC-MS ESI－

  • Received:2010-07-12 Revised:2010-09-06 Online:2010-10-28 Published:2010-10-28
  • Contact: WANG Yan-Hong

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摘要： 采用高效液相色谱质谱联用技术，在负离子扫描模式下建立了原料奶的乙腈提取成分的指纹图谱。采用乙腈和0.1％的乙酸为流动相进行二元梯度洗脱，柱温30℃，分析时间为85 min。确定了11个共有峰，以7号峰为参照物，通过相对峰面积和相对保留时间进行了方法学考察。结果表明，本方法具有良好的重现性，各指纹峰相对保留时间的RSD<0.79％，相对峰面积的RSD<2.84％。在原料奶指纹图谱基础上，选取有代表性有害物如防腐剂苯甲酸等进行了标准添加，建立了添加图谱，对沈阳地区超市的50个纯牛奶样品进行了筛查，取得初步应用结果。

关键词: 原料奶，指纹图谱，HPLC-MS

Abstract: The HPLC-MS fingerprint of raw milk extracted by acetonitrile was established to evaluate the quality of raw milk．A total of 11 peaks had been identified. The methodology was studied by the relative peak area and relative retention time with No. 7 peak as the reference. The results showed that the fingerprint established by this method has reasonable precision, reproducibility and stability with the relative standard deviations (RSDs) less than 0.79% for the relative retention time and less than 2.84% for the relative peak area．This method has provided a feasible analytical approach to explore the overall quality characteristics of raw milk.

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