色谱

色谱 ›› 2011, Vol. 29 ›› Issue (01): 59-62.DOI: 10.3724/SP.J.1123.2011.00059

• 研究论文 • 上一篇    下一篇

反相高效液相色谱法定量分析木质素的主要降解产物

江智婧, 朱均均, 李鑫, 连之娜, 余世袁, 勇强*   

  1. 南京林业大学 林木遗传与生物技术省部共建教育部重点实验室, 江苏 南京 210037
  • 收稿日期:2010-09-03 修回日期:2010-10-20 出版日期:2011-01-28 发布日期:2011-01-28
  • 通讯作者: 勇强,教授,主要从事生物质资源生物降解与转化的研究. E-mail: swhx@njfu.com.cn.
  • 基金资助:

    国家自然科学基金项目(30871992)、国家“863计划”(2008AA05Z401)和国家林业局公益性行业科研专项课题(201004001).

Determination of main degradation products of lignin using reversed-phase high performance liquid chromatography

JIANG Zhijing, ZHU Junjun, LI Xin, LIAN Zhina, YU Shiyuan, YONG Qiang*   

  1. Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
  • Received:2010-09-03 Revised:2010-10-20 Online:2011-01-28 Published:2011-01-28
  • Contact: Yong Qiang

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1117

被引次数

29

摘要： 建立了反相高效液相色谱定量分析玉米秸秆蒸汽爆破预处理过程中产生的主要木质素降解产物的方法。采用C18色谱柱,柱温30 ℃,乙腈-水(含1.5%的醋酸)为流动相,梯度洗脱,流速为0.8 mL/min, 254 nm和280 nm波长下紫外检测,可实现4-羟基苯甲酸、香草酸、紫丁香酸、4-羟基苯甲醛、香草醛和紫丁香醛的有效分离。6种主要木质素降解产物线性回归方程相关系数为0.9999～1.0000,加标回收率均在96%以上,相对标准偏差低于2.5%(n=6),满足定量分析要求。

关键词: 反相高效液相色谱, 酚类化合物, 降解产物, 木质素, 玉米秸秆

Abstract: An analytical method using reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the separation and quantitative determination of main degradation products of lignin (4-hydroxybenzoic acid, vanillic acid, syringic acid, 4-hydroxybenzaldehyde, vanillin and syringaldehyde) during the steam exploded pretreatment for corn stovers. The separation was carried out on a C18 column with the mobile phase of acetonitrile-water (containing 1.5% acetic acid) at 30 ℃ at a flow rate of 0.8 mL/min and the detection wavelengths of 254 and 280 nm. Under the optimized conditions, the correlation coefficients of the 6 compounds were between 0.9999 and 1.0000. The recoveries of the 6 compounds were all above 96% and the relative standard deviations (n=6) were less than 2.5%. This method is suitable for the determination of the main degradation products of lignin during the steam exploded pretreatment of lignocellulosics.

Key words: corn stovers, degradation products, lignin, phenolic compounds, reversed-phase high performance liquid chromatography (RP-HPLC)