色谱

色谱

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液相色谱-质谱联用的代谢组学用于中药通心络和人参对过度疲劳大鼠干预作用的评价

戴伟东1, 张凤霞1, 贾振华2, 魏聪2, 高鹏1, 路鑫1, 吴以岭2*, 许国旺1*   

  1. 1. 中国科学院分离分析化学重点实验室, 中国科学院大连化学物理研究所, 辽宁 大连 116023; 2. 河北省中西医结合医药研究院, 河北 石家庄 050035
  • 收稿日期:2011-08-15 修回日期:2011-10-17 出版日期:2011-11-28 发布日期:2011-12-25
  • 通讯作者: 许国旺,博士,研究员,主要研究方向为色谱及质谱技术的基础理论和应用研究,近年来尤其注重研究发展多维色谱和代谢组学. 吴以岭,教授,主任医师,主要研究方向为中医络病及心脑血管、糖尿病基础与临床.
  • 基金资助:

    国家自然科学基金重点项目(No. 20837003)和北京市卫生系统高层次卫生技术人才培养计划项目.

Evaluation of the effect of the traditional Chinese medicine Tongxinluo or ginseng on excess fatigue rats studied by metabonomics approach based on liquid chromatography-mass spectrometry

DAI Weidong1, ZHANG Fengxia1, JIA Zhenhua2, WEI Cong2, GAO Peng1, LU Xin1, WU Yiling2*, XU Guowang1*   

  1. 1. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. The Integration of Traditional and Western Medical Research Academy of Hebei Province, Shijiazhuang 050035, China
  • Received:2011-08-15 Revised:2011-10-17 Online:2011-11-28 Published:2011-12-25

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摘要： 用代谢组学方法评价了中药通心络和人参对过度疲劳大鼠的干预作用。通过构造大鼠过度疲劳模型,并分别用通心络和人参进行干预,采用快速液相色谱-离子阱飞行时间质谱(UFLC-IT-TOF-MS)获取大鼠血浆代谢轮廓,并用正交偏最小二乘法(OPLS)进行多变量统计分析,分别找出用于区分通心络和人参干预组大鼠同正常对照大鼠、过度疲劳大鼠的重要差异代谢物。结果显示过度疲劳大鼠体内的色氨酸、胆汁酸、溶血磷脂酰胆碱等代谢通路发生较大变化,经通心络或人参干预的大鼠整体代谢轮廓趋向正常水平,并能够部分调节上述发生变化的代谢通路使之往正常方向变化。

关键词: 代谢组学, 过度疲劳, 人参, 通心络, 液相色谱-质谱联用, 正交偏最小二乘法

Abstract: Excess fatigue is a pathological state of continuing accumulation of fatigue, which may cause the deterioration of body health, occurrence of diseases, and even lead to death. A metabonomics study was performed on the excess fatigue rats treated with traditional Chinese medicine Tongxinluo or ginseng based on ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry (UFLC-IT-TOF-MS). The plasma metabolic profiling data of the control rats, excess fatigue rats, and excess fatigue rats treated with Tongxinluo or ginseng were acquired. The orthogonal partial least squares analysis (OPLS) was applied for the multivariate statistics and the discovery of important differential metabolites distinguishing the excess fatigue rats treated with Tongxinluo or ginseng from the control rats and excess fatigue rats. The results showed tryptophan, bile acid, lysophosphatidylcholine metabolism were disturbed in the excess fatigue rats. The metabolic pattern including the related metabolic pathways of the rats, being treated with Tongxinluo or ginseng, was adjusted towards the normal state.

Key words: excess fatigue, ginseng, metabonomics, orthogonal partial least squares analysis (OPLS), Tongxinluo, liquid chromatography-mass spectrometry (LC-MS)