应用动态顶空吸附-气相色谱-质谱法分析算盘子花气味的化学成分

doi:10.3724/SP.J.1123.2014.11005

色谱 ›› 2015, Vol. 33 ›› Issue (3): 318-322.DOI: 10.3724/SP.J.1123.2014.11005

应用动态顶空吸附-气相色谱-质谱法分析算盘子花气味的化学成分

黄代红, 张振国, 陈国平, 李后魂, 石福臣

南开大学生命科学学院, 天津 300071

收稿日期:2014-11-05 修回日期:2014-12-15 出版日期:2015-03-08 发布日期:2015-02-12
通讯作者: 石福臣
基金资助:
国家自然科学基金重点项目(30930014).

Analysis of the components of floral scent in Glochidion puberum using gas chromatography-mass spectrometry with dynamic headspace adsorption

HUANG Daihong, ZHANG Zhenguo, CHEN Guoping, LI Houhun, SHI Fuchen

College of Life Science, Nankai University, Tianjin 300071, China

Received:2014-11-05 Revised:2014-12-15 Online:2015-03-08 Published:2015-02-12

摘要/Abstract

摘要：

花气味在维持大戟科(Phyllanthaceae)算盘子属(Glochidion)植物和头细蛾(Epicephala)专性传粉互利共生体系中发挥关键作用。本研究采用动态顶空吸附法(dynamic headspace adsorption)收集算盘子(Glochidion puberum)花气味,运用气相色谱-质谱(GC-MS)联用技术分离并鉴定其化学成分,用峰面积归一化法测得各组分的相对含量。结果表明:算盘子花气味中共鉴定出45种挥发物,主要包含单萜和倍半萜类物质;芳樟醇(38.06%)和β-榄香烯(23.84%)相对含量最高,占总相对含量的61.9%,为主要的气味成分,推测这两种物质可能是吸引传粉头细蛾的重要气味成分。本研究为进一步开展触角电生理检测和生物行为试验来筛选吸引传粉头细蛾的活性物质提供了理论依据。

关键词: 动态顶空吸附, 互利共生关系, 花气味, 气相色谱-质谱, 算盘子

Abstract:

The floral scent plays the important key role in maintaining the obligate pollination mutualism between Glochidion plants and Epicephala moths. In the study, the dynamic headspace adsorption technique was employed to collect the floral scent emitted by Glochidion puberum, gas chromatography coupled with mass spectrometry (GC-MS) was used for the detection and identification of volatile chemical components in headspace samples of flowers from G. puberum. The peak area normalization was used to determine the relative contents of each odour component. The results showed that 45 compounds mainly consisting of monoterpenes and sesquiterpenes were isolated from the floral scent produced by G. puberum. Especially, both linalool (38.06%) and β-elemene (23.84%) were considered as the major scent components of G. puberum. It was speculated that linalool and β-elemene may be the two potential compounds attracting female Epicephala moths. The study provided the basic data for further electroantennographic detection and bioassays to identify the compounds having the actual physiological activity to female Epicephala moths.

Key words: Glochidion puberum, dynamic headspace adsorption (DHSA), floral scent, gas chromatography-mass spectrometry (GC-MS), mutualism

中图分类号:

O658

黄代红, 张振国, 陈国平, 李后魂, 石福臣. 应用动态顶空吸附-气相色谱-质谱法分析算盘子花气味的化学成分[J]. 色谱, 2015, 33(3): 318-322.

HUANG Daihong, ZHANG Zhenguo, CHEN Guoping, LI Houhun, SHI Fuchen. Analysis of the components of floral scent in Glochidion puberum using gas chromatography-mass spectrometry with dynamic headspace adsorption[J]. Chinese Journal of Chromatography, 2015, 33(3): 318-322.

参考文献

[1] Kato M, Takimura A, Kawakita A. Proc Natl Acad Sci, 2003, 100(9): 5264
[2] Kawakita A, Kato M. Am J Bot, 2004, 91(3): 410
[3] Kawakita A, Kato M. Am J Bot, 2004, 91(9): 1319
[4] Kawakita A. Plant Spec Biol, 2010, 25: 3
[5] Zhang J, Wang S X, Li H H, et al. PLoS ONE, 2012, 7(7): e41657
[6] Hossaert-McKey M, Soler C, Schatz B, et al. Chemoecology, 2010, 20(2): 75
[7] Okamoto T, Kawakita A, Kato M. J Chem Ecol, 2007, 33: 1065
[8] Svensson G P, Okamoto T, Kawakita A, et al. New Phytol, 2010, 186: 995
[9] Schiestl F P. Ecol Lett, 2010, 13: 643
[10] Ding H J, Guo Y Y. Plant Protection (丁红建, 郭予元. 植物保护), 1995(5): 33
[11] Wei M, Deng X J, Hu W L, et al. Chinese Journal of Ecology (魏明, 邓晓军, 胡文利, 等. 生态学杂志), 2004, 23 (4): 184
[12] Agelopoulos N G, Pickett J A. J Chem Ecol, 1998, 24: 1161
[13] Li Q L, Ma X K, Cheng J, et al. Biodiversity Science (李庆良, 马晓开, 程瑾, 等. 生物多样性), 2012, 20 (3): 308
[14] Chen C, Song Q X, Proffit M, et al. Funct Ecol, 2009, 23: 941
[15] Li Z B, Yang P, Peng Y Q, et al. Chinese Journal of Tropical and Subtropical Botany (李宗波, 杨培, 彭艳琼, 等. 热带亚热带植物学报), 2012, 20(3): 292
[16] Li Z B, Yang P, Peng Y Q, et al. Chinese Journal of Yunnan University (李宗波, 杨培, 彭艳琼, 等. 云南大学学报), 2012, 34(1): 90
[17] Zhang H X, Hu Z H, Leng P S, et al. Scientia Agricultura Sinica (张辉秀, 胡增辉, 冷平生, 等. 中国农业科学), 2013, 46(4): 790
[18] Wang C Z, Su Y, Guo Y L. Chinese Journal of Organic Chemistry (王呈仲, 苏越, 郭寅龙. 有机化学), 2009, 29(6): 948
[19] Ding Q Q, Wu X B, Liu F, et al. Chinese Journal of Zhejiang A & F University (丁倩倩, 吴兴波, 刘芳, 等. 浙江农林大学学报), 2013, 30(4): 477
[20] Wang T B, Yan X, Lan X Z. Chinese Journal of Chromatography (王同波, 闫潇, 兰孝征. 色谱), 2013, 31(5): 467
[21] Chi S Y, Wu D K, Sun J H, et al. Chinese Journal of Chromatography (池舒耀, 伍迪科, 孙晋红, 等. 色谱), 2014, 32(5): 553
[22] Ye H Z, Li L. Chinese Journal of Chromatography (叶怀庄, 李莉. 色谱), 2005, 23(1): 109
[23] Li B T. Flora of China. Beijing: Science Press (李秉滔. 中国植物志. 北京: 科学出版社), 1994, 44 (1): 68
[24] Hu B B, Li H H, Shi F C. Acta Zootaxonomica Sinica (胡冰冰, 李后魂, 石福臣. 动物分类学报), 2011, 36(2): 447
[25] Raguso R A, Pellmry O. Oikos, 1998, 81: 238
[26] Shuttleworth A, Johnson S D. Proc R Soc B, 2010, 277: 2811
[27] Knudsen J T, Eriksson R, Gershenzon J, et al. Bot Rev, 2006, 72: 1
[28] Pare P, Tumlinson J H. J Plant Physiol, 1997, 114: 1161
[29] Kawakita A, Kato M. Proc R Soc B, 2009, 276: 417

应用动态顶空吸附-气相色谱-质谱法分析算盘子花气味的化学成分

Analysis of the components of floral scent in Glochidion puberum using gas chromatography-mass spectrometry with dynamic headspace adsorption

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

扫码分享

参考文献

相关文章 15

编辑推荐

Metrics

[1]	王亚婷, 杨阳, 孙秀兰, 纪剑. 基于气相色谱-质谱法的广泛靶向代谢组学方法开发[J]. 色谱, 2023, 41(6): 520-526.
[2]	吴瑛瑕, 牟燕, 刘佩珊, 张易天, 曾颖炫, 周枝凤. 气相色谱-质谱法测定大鼠肝脏中的39种脂肪酸[J]. 色谱, 2023, 41(5): 443-449.
[3]	陈佳, 刘玉龙, 徐斌, 刘勤, 谢剑炜. 顶空固相微萃取-气相色谱-质谱法快速筛查油性基质中化学武器公约相关化合物[J]. 色谱, 2023, 41(4): 348-358.
[4]	吴丽娟, 杨丽莉, 胡恩宇, 王美飞, 杨超, 尹明明. 气相色谱-质谱法测定土壤中14种苯胺类和联苯胺类化合物[J]. 色谱, 2023, 41(12): 1127-1134.
[5]	胡国绅, 王红, 余可垚, 沈伟健, 侯燕, 季美泉, 朱一鸣, 田雯, 李锡东. 气相色谱-负化学源质谱法测定船舶压载水中4种三卤甲烷[J]. 色谱, 2022, 40(6): 584-589.
[6]	穆应花, 邢家溧, 沈坚, 应璐, 毛玲燕, 徐晓蓉, 娄永江, 吴希. QuEChERS-气相色谱-质谱法检测鱼肉中19种氯酚类化合物[J]. 色谱, 2022, 40(5): 477-487.
[7]	王雪婷, 李静静, 姜珊, 沈伟健, 王毅谦, 顾强. 酸性酯交换-气相色谱-质谱法同时测定植物油中氯丙二醇酯和缩水甘油酯[J]. 色谱, 2022, 40(2): 198-205.
[8]	李晓森, 吴姬娜, 夏俊美, 袁铃, 杨旸. 基于巯基化衍生的气相色谱-质谱法测定有机相及水相中氯化氰[J]. 色谱, 2021, 39(8): 913-920.
[9]	刘玲玲, 张利钧, 董希良, 陈晓梅, 赵传明. 液液萃取-气相色谱-质谱法同时测定水中46种半挥发性有机物[J]. 色谱, 2021, 39(5): 510-517.
[10]	吕稳, 李红英, 刘杰, 韩玮, 黄伟. 气相色谱-质谱法测定化妆品中13种防晒剂[J]. 色谱, 2021, 39(5): 552-557.
[11]	汤娟, 费晓庆, 周佳, 钱凯, 董绍伟, 曹丽华, 丁友超. 气相色谱-质谱法同时测定化妆品中18种氯代烃类有机溶剂[J]. 色谱, 2021, 39(3): 324-330.
[12]	王枫雅, 冯亮. 硼酸官能化金属-有机骨架磁性纳米复合材料的制备及其在茶叶农药残留检测中的应用[J]. 色谱, 2021, 39(10): 1111-1117.
[13]	高瑶, 龚思思, 张天闻, 唐敏洁, 张蓓英, 陈敏, 欧启水, 毛厚平. 基于气相色谱-质谱联用技术的血清氨基酸代谢谱在尿石症中的应用[J]. 色谱, 2020, 38(5): 587-594.
[14]	沈伟健, 王红, 陆慧媛, 余可垚, 胡国绅, 魏雪缘, 吴斌. 气相色谱-质谱法测定植物油中8种维生素E及其在芝麻油真伪鉴别方面的应用[J]. 色谱, 2020, 38(5): 595-599.
[15]	刘静, 黄青丹, 王勇, 曾炼, 张亚茹. 基于自制气体循环系统测定全氟异丁腈中杂质成分[J]. 色谱, 2020, 38(5): 606-610.