色谱

色谱

• 研究论文 • 上一篇    下一篇

反相高效液相色谱法同时测定墨旱莲中的蟛蜞菊内酯和异去甲基蟛蜞菊内酯

原红霞,赵云丽,王晓英,唐倩,高晓霞,于治国

  

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • 收稿日期:2006-10-19 修回日期:2006-11-20 出版日期:2007-05-30 发布日期:1985-09-25
  • 通讯作者: 于治国

Simultaneous Determination of Wedelolactone and Isodemethyl-wedelolactone in Herba Ecliptae by Reversed-Phase
High Performance Liquid Chromatography

YUAN Hongxia, ZHAO Yunli, WANG Xiaoying, TANG Qian, GAO Xiaoxia, YU Zhiguo   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2006-10-19 Revised:2006-11-20 Online:2007-05-30 Published:1985-09-25

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摘要：

建立了同时测定墨旱莲中香豆草醚类成分蟛蜞菊内酯和异去甲基蟛蜞菊内酯含量的高效液相色谱法。采用Kromasil C18柱(250 mm×4.6 mm, 5 μm),流动相为甲醇-0.5%醋酸水溶液(体积比为55∶45),流速为1.0 mL/min,检测波长为351 nm,柱温为30 ℃,进样量为20 μL。在上述条件下测得的异去甲基蟛蜞菊内酯和蟛蜞菊内酯的质量浓度分别在1.6~32.0 mg/L和5.6~112.0 mg/L时与色谱峰面积之间的线性关系良好,二者高、中、低浓度条件下的平均加标回收率分别为97.5%～ 98.2%和99.0%～ 100.2%。该方法简便、快速、准确,可作为墨旱莲质量控制的一个有效方法。

关键词: 高效液相色谱法, 墨旱莲 , 蟛蜞菊内酯, 异去甲基蟛蜞菊内酯

Abstract:

A reversed-phase high performance liquid chromatographic method (RP-HPLC) was developed for the simultaneous determination of wedelolactone and isodemethylwedelolactone in Herba Ecliptae from different sources. The separation was performed on a Kromasil C18 column (250 mm×4.6 mm, 5 μm), with methanol-0.5% acetic acid (55∶45, v/v) as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was set at 351 nm and the volume of injection was 20 μL. There were good linear relationships between the mass concentrations and the peak areas of isodemethylwedelolactone and wedelolactone in the ranges of 1.6-32.0 mg/L (r=0.9995) and 5.6-112.0 mg/L (r=0.9998), respectively. The recoveries were found to be in the range of 97.5%-98.2% and 99.0%-100.2%, respectively. The results of the experiments have demonstrated that the established method is rapid and simple with good accuracy and reproducibility for the simultaneous determination of wedelolactone and isodemethylwedelolactone. The method is suitable for the quality control of Herba Ecliptae from different sources.

Key words: Herba Ecliptae , isodemethylwedelolactone, wedelolactone, reversed-phase high performance liquid chromatography (RP-HPLC)