关键词: 高效液相色谱, 农药残留, 生态纺织品 , 有机氯农药

Abstract:

A method for the simultaneous determination of pesticide and herbicide residues in cotton textile by high performance liquid chromatography coupled with diode array detector (HPLC-DAD) has been established. The pesticides and herbicides determined were 2,4-dichlorophenoxy (2,4-D), 2-methyl-4-chlorophenoxy acetic acid (MCPA), dichlorprop, mecoprop, 2,4,5-trichlorophenoxy (2,4,5-T), 2-methyl-4-chlorophenoxy butyric acid (MCPB), β-endosulfan, methoxychlor and 2,2-bis(p-chlorophenyl)-1,1-dichloroethene (DDE). Pesticide and herbicide residues in cotton products were extracted with acetone-petroleum ether by ultrasonic instrument. The extract was analyzed using an SGE C18 column with a linear gradient elution (methanol-phosphoric acid as mobile phase at a flow rate of 0.8 mL/min) and ultraviolet detection at 230 nm. The linear range was 0.5-10 mg/L, and the correlation coefficients were all greater than 0.9988. By using acetone-petroleum ether(4∶1, v/v) as solvent, the recoveries of nine organochlorine pesticides and herbicides at spiked levels of 0.5-2 mg/kg were 85.5%-99.6%. The limits of detection (S/N=3) were 0.05 mg/kg except MCPB, β-endosulfan, methoxychlor and DDE that were 0.10 mg/kg. This method is of high accuracy, easy operation, satisfactory recoveries and good reproducibility. It could be used for the determination of the nine pesticides and herbicides in textile.

Key words: eco-textile , organochlorine pesticides, pesticide-residues, high performance liquid chromatography (HPLC)