色谱

色谱

• 研究快报 • 上一篇    下一篇

胶束电动色谱法和微柱液相色谱法分析白藜芦醇的比较

范恩国1,3,黄晓晶2,张锴1,白玉华1,高如瑜1,阎超2

  

  1. 1.College of Life Sciences, Nankai University, Tianjin 300071, China; 2.Dalian Institute of Chemical Physics,
    Chinese Academy of Sciences, Dalian 116023, China; 3.Institute of Plant and Cell Physiology,
    Martin-Luther University Halle-Wittenberg, Weinbergweg 10, 06120 Halle/Saale, Germany
  • 收稿日期:2006-08-21 修回日期:2007-04-20 出版日期:2007-05-30 发布日期:1985-09-25
  • 通讯作者: 阎超

Comparison of Micellar Electrokinetic Chromatography and Microcolumn High Performance Liquid
Chromatography for Resveratrol Analysis

FAN Enguo1,3, HUANG Xiaojing2, ZHANG Kai1, BAI Yuhua1, GAO Ruyu1, YAN Chao2   

  1. 1.College of Life Sciences, Nankai University, Tianjin 300071, China; 2.Dalian Institute of Chemical Physics,
    Chinese Academy of Sciences, Dalian 116023, China; 3.Institute of Plant and Cell Physiology,
    Martin-Luther University Halle-Wittenberg, Weinbergweg 10, 06120 Halle/Saale, Germany
  • Received:2006-08-21 Revised:2007-04-20 Online:2007-05-30 Published:1985-09-25

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摘要： 白藜芦醇可以顺式和反式两种形式存在。它是一种具有药理活性的植物抗毒素,具有抗肿瘤、保护心血管、防止机体老化的功效。分别采用胶束电动色谱和微柱液相色谱两种方法对白藜芦醇的两种异构体进行了分离,并对这两种方法进行了比较。结果表明,两种方法均具有分离快速、重现性好、溶剂消耗少的特点,都适用于白藜芦醇的分析。

关键词: 白藜芦醇, 胶束电动色谱, 方法比较
, 微柱液相色谱, 异构体

Abstract: Resveratrol, occurring in trans- and cis-isomeric forms, is a phytoalexin with pharmacological activities, for example, anti-cancer, anti-cardiovascular disease, and life-span increasing properties. Two analytical methods, namely, micellar electrokinetic chromatography (MEKC) and microcolumn high performance liquid chromatography (μ-HPLC), were developed and compared for resveratrol isomer separation. Both methods are suitable for resveratrol analysis owing to their performances of high speed, good reproducibility， and low consumption of solvents and samples.

Key words: isomers, method comparison , micellar electrokinetic chromatography (MEKC), resveratrol, microcolumn high performance liquid chromatography (μ-HPLC)