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色谱

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小型化毛细管电泳-电化学检测法测定猪尿和猪饲料中的β-兴奋剂

王伟宇,张玉莲,邢晓平,王金妍,石雪,叶建农

  

  1. Department of Chemistry, East China Normal University, Shanghai 200062, China
  • 收稿日期:2007-07-16 修回日期:2007-09-18 出版日期:2008-03-30 发布日期:1984-06-25
  • 通讯作者: 叶建农

Determination of β-adrenergic agonists in pig urine and pig fodder using miniaturized capillary electrophoresis
with electrochemical detection

WANG Weiyu, ZHANG Yulian, XING Xiaoping, WANG Jinyan, SHI Xue, YE Jiannong

  

  1. Department of Chemistry, East China Normal University, Shanghai 200062, China
  • Received:2007-07-16 Revised:2007-09-18 Online:2008-03-30 Published:1984-06-25

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摘要：

采用小型化毛细管电泳-电化学检测技术测定了猪尿和猪饲料中的克伦特罗(clenbuterol,俗称瘦肉精)及其替代品莱克多巴胺(ractopamine)和沙丁胺醇(salbutamol)。考察了工作电极的氧化电位、运行缓冲液的酸度和浓度、分离电压和进样时间等因素对分离和检测的影响。以直径300 μm的碳圆盘电极为工作电极,检测电极电位为+0.95 V (vs SCE),在100 mmol/L的硼酸盐(pH 9.15)运行缓冲液中,上述3种组分在7 min内实现了较好的分离。被测物浓度与峰电流在3个数量级范围内呈良好的线性关系,检测限为1.20×10-7~2.06×10-7 g/mL。该方法简单可靠,已成功应用于猪尿和猪饲料样品中3种β-兴奋剂的测定,是一种有效的食品安全分析检测方法。

关键词: 克伦特罗, 莱克多巴胺, 沙丁胺醇, 小型化毛细管电泳-电化学检测法, 猪尿, 猪饲料

Abstract:

A method of miniaturized capillary electrophoresis with electrochemical detection has been developed for the determination of β-adrenergic agonists in pig urine and pig fodder. Several important factors, including running buffer acidity, separation voltage, working electrode potential, etc., were evaluated to acquire optimum analysis conditions. Under the selected optimum conditions, these analytes can be well separated in 7 min. Good linear relationship was established between the peak current and the concentration of each analyte over 3 orders of magnitude. The detection limits ranged from 1.20×10-7 to 2.06×10-7 g/mL (S/N=3). The proposed method has been successfully applied for the determination of β-adrenergic agonist in pig urine and pig fodder with satisfactory results, providing a useful monitoring method for food safety.

Key words: clenbuterol, pig fodder , pig urine, ractopamine, salbutamol, miniaturized capillary electrophoresis-electrochemical detection