色谱

色谱

• 研究论文 • 上一篇    下一篇

重组人干扰素-γ的制备与鉴定

吴丹,高栋,白泉,耿信笃

  

  1. Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Northwest University, Xi’an 710069, China
  • 收稿日期:2007-12-03 修回日期:1900-01-01 出版日期:2008-03-30 发布日期:1984-06-25
  • 通讯作者: 耿信笃

Preparation and identification of recombinant human interferon-γ


WU Dan, GAO Dong, BAI Quan, GENG Xindu

  

  1. Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Northwest University, Xi’an 710069, China
  • Received:2007-12-03 Revised:1900-01-01 Online:2008-03-30 Published:1984-06-25
  • Contact: Geng Xin-du

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摘要：

用聚乙二醇200疏水相互作用色谱固定相(PEG200-STHIC)分别在色谱柱和色谱饼上完成了一步复性并同时纯化来源于大肠杆菌(E.coli)表达的重组人干扰素-γ(rhIFN-γ)。为了能使色谱分离方法用于不同来源的rhIFN-γ的纯化,对rhIFN-γ在反相色谱、离子交换色谱、固定化镍离子亲和色谱上的保留行为也进行了研究。色谱柱纯化的rhIFN-γ收集液经排阻色谱除盐和冷冻干燥得到rhIFN-γ干粉。用基质辅助激光解吸电离飞行时间质谱对rhIFN-γ干粉进行了测定,rhIFN-γ单体的相对分子质量为17184.0,二聚体的相对分子质量为34204.4。用细胞病变抑制法(CPEI)测定rhIFN-γ干粉的比活性为9.5×108 IU/mg。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测定rhIFN-γ干粉的纯度高于95%。用色谱柱复性并同时纯化rhIFN-γ的质量回收率达到93.7%,纯度高于95%,比活性为4.3×107 IU/mg。结果表明,采用PEG200-STHIC色谱柱复性并同时纯化rhIFN-γ是一种十分高效的方法。

关键词: 纯化 , 蛋白折叠液相色谱法, 复性, 高效疏水色谱, 重组人干扰素-γ

Abstract:

The renaturation with simultaneous purification of recombinant human interferon-γ (rhIFN-γ) expressed as inclusion bodies in Escherichia coli (E.coli) was accomplished by the stationary phase of hydrophobic interaction chromatography (STHIC) with the end group of poly(ethylene glycol) （PEG）(PEG200) packed in a chromatographic column and a chromatographic pie by nonlinear gradient, separately. In order to provide more selections for the chromatographic separation of rhIFN-γ from different sources, the chromatographic behavior of rhIFN-γ in reversed-phase liquid chromatography, ion-exchange chromatography and immobilized-nickel affinity chromatography were also studied. The fraction of the renatured and purified rhIFN-γ from HIC was desalted by the size exclusion chromatography, subsequently freeze-dried to powder. With matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), monomeric and dimeric rhIFN-γ were found in the powder due to the freeze-dried process and their relative molecular masses were 17184.0 and 34204.4, respectively. With the bioactivity assay by cytopathic effect inhibition (CPEI), the specific bioactivity of rhIFN-γ was 9.5×108 IU/mg, which was higher than that of the required criteria in the pharmacopoeia of China, because the presence of dimeric rhIFN-γ which has much higher specific bioactivity than its monomer in the powder. The obtained mass recovery, purity, specific bioactivity of the purified monomeric rhIFN-γ were 93.7%, ＞95%, and 4.3×107 IU/mg, respectively. The results showed that the renaturation with simultaneous purification of rhIFN-γ by PEG200-STHIC is a kind of efficient method.

Key words: high performance hydrophobic interaction chromatography (HPHIC), protein folding liquid chromatography (PFLC), purification , renaturation, recombinant human interferon-γ (rhIFN-γ)