色谱

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魔芋的抗病性与叶片中水溶性氨基酸含量的关系

陈永波,降巧龙

  

  1. The Konjac Research Institute of Enshi Autonomic State Academy of Agricultural Sciences, Enshi 445000, China
  • 收稿日期:2007-09-18 修回日期:2007-11-17 出版日期:2008-05-30 发布日期:1984-03-25
  • 通讯作者: 陈永波

Relationship of resistance to diseases and water-soluble amino acids in Konjac leaves

CHEN Yongbo, JIANG Qiaolong   

  1. The Konjac Research Institute of Enshi Autonomic State Academy of Agricultural Sciences, Enshi 445000, China
  • Received:2007-09-18 Revised:2007-11-17 Online:2008-05-30 Published:1984-03-25
  • Contact: CHEN Yongbo

摘要:

利用反相高效液相色谱法(RP-HPLC)收集了正常花魔芋、软腐病花魔芋、白魔芋和珠芽魔芋叶片的水溶性氨基酸图谱,分析了对
软腐病具有不同抗病性的魔芋品种与多种水溶性氨基酸含量和比例的关系。结果表明:不同抗病性的品种及同一品种的正常魔芋叶片
和病株魔芋叶片中水溶性氨基酸的含量存在显著差别,因而可以以此方法为基础建立指纹图谱库,分析特征峰与魔芋抗病性的关系,为
研究魔芋病害防治技术提供新的思路。

关键词: 反相高效液相色谱法, 抗病性 , 魔芋叶片, 水溶性氨基酸

Abstract:

Reversed-phase high performance liquid chromatography was used to analyze water-soluble amino acids in the
normal Amorphophallus Konjac, Amorphophallus albus, Amorphophallus bulbifer, and the soft rot Amorphophallus Konjac,
to determine the relationship of the different soft-rot resistant Konjac varieties and the proportion and content of
the multiple water-soluble amino acids. The results showed that there are remarkable differences in the content and
proportion of water-soluble amino acids in different resistant varieties and the same variety of normal and diseased
leaves of Amorphophallus. In this study, the bank of fingerprint chromatogram was established and can be used to
analyze the related characteristic peaks and the resistance of Amorphophallus.

Key words: Konjac leaf blade, resistance , water-soluble amino
acids,
reversed-phase high performance liquid chromatography (RP-HPLC)