色谱

色谱 ›› 2008, Vol. 26 ›› Issue (3): 314-317.

• 研究论文 • 上一篇    下一篇

全扫描-选择离子数据采集技术应用于烟草中生物碱的快速测定

王保兴;杨式华;侯英;曾晓鹰;武怡;许国旺   

  1. 1.Yunnan Reascend Tobacco Technology (Group) Co., Ltd, Kunming 650106, China;
    2.Hongyun Tobacco (Group) Co., Ltd, Kunming 650202, China;
    3.National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China
  • 收稿日期:2007-08-15 修回日期:2007-11-01 出版日期:2008-05-30 发布日期:1984-03-25
  • 通讯作者: 许国旺

Rapid determination of alkaloids in tobacco using gas chromatography-mass spectrometry with full scan-selected ion monitoring mode

WANG Baoxing; YANG Shihua;HOU Ying;ZENG Xiaoying;WU Yi;XU Guowang   

  1. 1.Yunnan Reascend Tobacco Technology (Group) Co., Ltd, Kunming 650106, China;
    2.Hongyun Tobacco (Group) Co., Ltd, Kunming 650202, China;
    3.National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China
  • Received:2007-08-15 Revised:2007-11-01 Online:2008-05-30 Published:1984-03-25

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摘要： 建立了应用气相色谱-质谱(GC-MS)全扫描-选择离子监测(Scan-SIM)数据采集方式同时测定烟草中8种含量相差较大的生物碱的方法,采用Scan模式分析烟碱、降烟碱、新烟碱、去氢新烟碱,同时采用SIM模式分析麦斯明、二烯烟碱、2,3′-联吡啶、可铁宁。结果表明,烟草中各生物碱的回收率为94.8%～98.8%,5次测定的相对标准偏差均小于6%。该方法具有简单、快速、准确的特点,应用于烟草样品测定,结果令人满意。

关键词: 气相色谱-质谱, 全扫描-选择离子监测, 生物碱, 烟草

Abstract: Simultaneous Scan-Selected ion monitoring mode (Scan-SIM) is a new improvement method of data acquisition of gas chromatography mass spectrometry (GC/MS). The determination method of alkaloids in tobacco was established by Scan-SIM of GC/MS. Nicotine, nornicotine, anabasine and anatabine were determined with Scan mode, and myosmine, nicotyrine, 2,3'-Bipyridyl and cotinine were determined with SIM mode. The average recoveries of alkaloids ranged from 94.8% to 98.8％ and relative standard deviations (RSDs) were less than 6.0% (n=5). Therefore, it is a simple, rapid, accurate method. Tobacco samples in different years were determined with this method, and the results were satisfactory.

Key words: alkaloids, full scan-selected ion monitoring mode （Scan-SIM）, gas chromatography-mass spectrometry （GC-MS）, tobacco