色谱

色谱

• 技术与应用 • 上一篇    

气相色谱-微池电子捕获器检测尿液中的三唑仑

覃华菁   

  1. Liuzhou Center for Disease Control and Prevention, Liuzhou 545001, China
  • 收稿日期:2007-09-13 修回日期:2008-01-08 出版日期:2008-05-30 发布日期:1984-03-25
  • 通讯作者: 覃华菁

Determination of triazolam in human urine by gas chromatograph-micro electron capture detector

QIN Huajing   

  1. Liuzhou Center for Disease Control and Prevention, Liuzhou 545001, China
  • Received:2007-09-13 Revised:2008-01-08 Online:2008-05-30 Published:1984-03-25

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摘要：

建立了气相色谱-微池电子捕获检测器(GC-μECD)检测尿液中三唑仑的方法。筛选了pH值、提取溶剂、涡旋时间,优化了液-液萃
取条件。在0.2～50 ng/mL范围内,线性关系良好,相关系数为0.9995；方法的检出限为0.1 ng/mL,日内和日间测定的相对标准偏差分
别为4.17%和5.31%,平均回收率为93.9%。该方法操作简便、灵敏度高、线性范围广、回收率高,完全能够满足日常检测工作的需要。

关键词: 尿液 , 气相色谱, 三唑仑, 微池电子捕获检测器

Abstract:

Triazolam is often abused in criminal activities. It is necessary to develop a simple, fast and sensitive
method for determining triazolam in human urine. After the triazolam in urine was extracted with ether at pH 9, the
analysis was performed on an HP-5 capillary column with gas chromatograph-micro electron capture detector (GC-
μECD). The calibration curve of triazolam showed good linearity in the range of 0.2-50 ng/mL with the correlation
coefficient of 0.9995. The inter-day relative standard deviation (RSD) and intra-day RSD were 4.17% and 5.31%,
respectively. The average recovery was 93.9%. This simple, sensitive and accurate method can be easily implemented
for monitoring triazolam in urine.

Key words: human urine , micro electron capture detector (μECD), triazolam, gas chromatography (GC)