色谱

色谱

• 专论与综述 • 上一篇    下一篇

整体柱制备技术的新进展及其在蛋白质组学中的应用

邹汉法*, 吴明火, 王方军, 吴仁安, 叶明亮   

  1. National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China
  • 收稿日期:2009-08-27 修回日期:2009-09-02 出版日期:2009-09-30 发布日期:1982-03-25
  • 通讯作者: 邹汉法

Recent developments in preparation of monolithic columns and their applications in proteomic analysis

ZOU Hanfa*, WU Minghuo, WANG Fangjun, WU Ren’an, YE Mingliang   

  1. National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China
  • Received:2009-08-27 Revised:2009-09-02 Online:2009-09-30 Published:1982-03-25
  • Contact: ZOU Hanfa

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1052

被引次数

85

摘要： 整体柱是通过在柱管内原位聚合或固化的方法制备得到的具有多孔结构的整体棒状固定相,与传统的填充柱相比,具有通透性好、传质速率快、容易制备等优点,因此在分离分析领域特别是生物分离分析中发挥的作用日益增大。整体柱的制备及应用近年来也得到了快速发展,层出不穷的新型整体柱已被广泛用于色谱高效分离分析、固相萃取及酶反应器等方面,大大推动了分离分析科学的发展。本文主要总结了近五年来整体柱的制备技术及其在蛋白质组学应用中的一些最新研究进展。

关键词: 蛋白质组学, 色谱分离, 整体柱, 制备, 综述

Abstract: Monolithic column is a continuous unitary porous structure prepared by in-situ polymerization or consolidation inside the column tubing. Monolithic columns have been attracted great attention due to their simple preparation procedure, high permeability and high mass transfer rate. Big progress on the technology and methods for the preparation of monolithic columns and their applications to the highly efficient separation and pretreatment of biological samples have been made in last decade. This review summarizes the developments in the preparation of the monolithic columns and their applications to the proteomic analysis during the last five years.

Key words: chromatographic separation, preparation, proteomic analysis, review , monolithic columns