色谱

色谱

• 专论与综述 • 上一篇    下一篇

分离分析技术在蛋白质组学研究中应用的新进展

张养军1, 张万军1, 马岩2, 彭博1, 钱小红1*   

  1. 1.State Key Laboratory of Proteomics, Beijing Institute of Radiation Medicine, Beijing Proteome Research Center, Beijing 102206, China; 2.Beijing University of Technology, Beijing 100022, China
  • 收稿日期:2009-09-01 修回日期:2009-09-04 出版日期:2009-09-30 发布日期:1982-03-25
  • 通讯作者: 钱小红

Advances of separation and analytical techniques in proteomic studies

ZHANG Yangjun1, ZHANG Wanjun1, MA Yan2, PENG Bo1, QIAN Xiaohong1*   

  1. 1.State Key Laboratory of Proteomics, Beijing Institute of Radiation Medicine, Beijing Proteome Research Center, Beijing 102206, China; 2.Beijing University of Technology, Beijing 100022, China
  • Received:2009-09-01 Revised:2009-09-04 Online:2009-09-30 Published:1982-03-25
  • Contact: QIAN Xiaohong

PDF

1075

被引次数

32

摘要： 蛋白质组学研究的核心技术之一是分离分析方法。该综述重点评述了分离分析技术在蛋白质组学研究,即在蛋白质组表达谱构建、翻译后修饰蛋白质组研究、蛋白质复合体和相互作用研究、蛋白质组定量研究中应用的新进展,介绍了各种分离分析方法的优点、应用范围和有待解决的问题。引用文献89篇。

关键词: 蛋白质相互作用 , 蛋白质组学, 定量技术, 分离技术, 质谱

Abstract: The separation and analytical technique is one of the core technologies in proteomic studies. The advances in the applications of separation and analytical techniques in proteomic studies on protein expression profiling, protein modification profiling, protein complex and interaction network as well as proteome quantitation are reviewed, and 89 references are cited.

Key words: mass spectrometry, protein-protein interaction , quantitation, separation technique, proteomics