色谱

色谱

• 专论与综述 • 上一篇    下一篇

免疫亲和毛细管电泳的研究进展

陈泓序, 张新祥*   

  1. Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
  • 收稿日期:2009-06-28 修回日期:2009-07-15 出版日期:2009-09-30 发布日期:1982-03-25
  • 通讯作者: 张新祥

Development of immunoaffinity capillary electrophoresis

CHEN Hongxu, ZHANG Xinxiang*   

  1. Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
  • Received:2009-06-28 Revised:2009-07-15 Online:2009-09-30 Published:1982-03-25
  • Contact: ZHANG Xinxiang

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716

被引次数

5

摘要： 免疫亲和毛细管电泳方法结合了免疫分析的高特异性和毛细管电泳分离的高效、快速、样品用量少等优点,是复杂样品中特定组分分析的重要方法之一。激光诱导荧光检测器的使用以及毛细管电泳分离前免疫预富集过程的引入,可以进一步提高分析测定的灵敏度,使其能够用于痕量物质的高灵敏测定。本文结合作者所在课题组的工作,对免疫亲和毛细管电泳的两种主要模式,即均相的毛细管电泳免疫分析(CEIA)和非均相的免疫亲和毛细管电泳(IACE)的研究进展进行了综述。

关键词: 毛细管电泳, 免疫分析, 免疫亲和, 综述

Abstract: Immunoaffinity capillary electrophoresis is one of the important analytical technologies for complicated samples. It combines the advantages of both immunoassay and capillary electrophoresis, such as high specificity, high separation efficiency, rapid and low consumption. With the aid of laser-induced fluorescence detector and immuno-enrichment, the sensitivity of the method can meet the requirement for trace detection. In this paper, the research progress of homogenous capillary electrophoresis immunoassay (CEIA) and immunoaffinity capillary electrophoresis (IACE) is reviewed based on the research of the author’s group.

Key words: immunoaffinity, immunoassay, review , capillary electrophoresis (CE)