色谱

色谱

• 研究论文 • 上一篇    下一篇

苏丹红I分子印迹聚合物的制备及其性能评价

戴晴, 王妍, 包学伟, 荆涛, 郝巧玲, 周宜开, 梅素容*   

  1. Key Laboratory of Environment & Health of Ministry of Education, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
  • 收稿日期:2009-03-12 修回日期:2009-05-12 出版日期:2009-11-30 发布日期:1981-12-25
  • 通讯作者: 梅素容

Preparation and evaluation of Sudan Red I molecularly imprinted polymer

DAI Qing, WANG Yan, BAO Xuewei, JING Tao, HAO Qiaoling, ZHOU Yikai, MEI Surong*   

  1. Key Laboratory of Environment & Health of Ministry of Education, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
  • Received:2009-03-12 Revised:2009-05-12 Online:2009-11-30 Published:1981-12-25
  • Contact: MEI Surong

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摘要： 以苏丹红I为模板分子,通过沉淀聚合法制备了一种对苏丹红I具有特异性吸附的分子印迹聚合物。通过选择性评价和前沿色谱实验,评价了致孔剂的选择和用量、功能单体和模板分子的物质的量比对分子印迹聚合物识别性能的影响。实验结果表明: 当以甲醇和乙腈的混合液(体积比为30:10)为致孔剂,甲基丙烯酸(MAA)为功能单体,且功能单体和模板分子的物质的量比为8:1时,分子印迹聚合物的印迹因子为2.32,亲和位点总数(Bt)为0.50 μmol/g;将其作为固相萃取柱填料用于辣椒粉样品中痕量苏丹红I的净化和富集,结果表明: 苏丹红I浓度在10～500 μmol/L范围内时,呈现良好的线性关系(r=0.999);检出限为3.3 μmol/L,加标回收率为95.87%～98.41%,相对标准偏差低于3.1%。该方法有望用于辣椒粉样品中苏丹红I添加剂的常规检测。

关键词: 沉淀聚合法, 分子印迹聚合物, 辣椒粉 , 苏丹红I

Abstract: The molecularly imprinted polymer (MIP) was prepared by precipitation polymerization using Sudan Red I as the template. To investigate the influence of porogenic solvent and the amount of template on recognition property, selective chromatographic evaluation and frontal chromatography were performed. The results indicated that the obtained MIP had the best affinity and selectivity to the template when the molar ratio of template to functional monomer was 1:8 and a mixture of 30 mL methanol and 10 mL acetonitrile was used as the porogenic solvent. The imprinted factor of optimal MIP for Sudan Red I was 2.32, and the total amount of the immobilized ligand was 0.50 μmol/g. This MIP displayed good specific recognition property for Sudan Red I and was used as the sorbent of solid phase extraction (SPE) to determine trace Sudan Red I in chili powders. The linear range was from 10 to 500 μmol/L with a correlation coefficient of 0.999. The detection limit was 3.3 μmol/L, the spiked recovery was between 95.87% and 98.41%, and the relative standard deviation (RSD) was lower than 3.1%. The developed method can be used for the routine detection of Sudan Red I in chili powders.

Key words: chili powders , precipitation polymerization, Sudan Red I, molecularly imprinted polymer (MIP)