色谱

色谱

• 研究论文 • 上一篇    下一篇

超高效液相色谱-电喷雾串联质谱法分离鉴定烟草中5种糖苷类香味前体物质

吴新华1,2, 朱瑞芝1,3*, 任卓英1, 王凯1,3, 牟定荣1, 魏万之3, 缪明明4   

  1. 1.Research and Development Center of Hongta Tobacco (Group) Co., Ltd, Yuxi 653100, China; 2.Kunming University of Science and Technology, Kunming 650224, China; 3.School of Chemistry and Chemical Engineering of Hunan University, Changsha 410082, China; 4.Yunnan Academy of Tobacco Science, Kunming 650106, China
  • 收稿日期:2009-03-31 修回日期:2009-05-31 出版日期:2009-11-30 发布日期:1981-12-25
  • 通讯作者: 朱瑞芝

Separation and identification of 5 glycosidic flavor precursors in tobacco by ultra performance liquid chromatography- electrospray ionization tandem mass spectrometry

WU Xinhua1,2, ZHU Ruizhi1,3*, REN Zhuoying1, WANG Kai1,3, MOU Dingrong1, WEI Wanzhi3, MIAO Mingming4   

  1. 1.Research and Development Center of Hongta Tobacco (Group) Co., Ltd, Yuxi 653100, China; 2.Kunming University of Science and Technology, Kunming 650224, China; 3.School of Chemistry and Chemical Engineering of Hunan University, Changsha 410082, China; 4.Yunnan Academy of Tobacco Science, Kunming 650106, China
  • Received:2009-03-31 Revised:2009-05-31 Online:2009-11-30 Published:1981-12-25
  • Contact: ZHU Ruizhi

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700

被引次数

23

摘要： 采用超高效液相色谱-电喷雾串联质谱法(UPLC-ESI MS/MS)并结合气相色谱-质谱法分离鉴定了烟草中5种主要的糖苷类香味前体物质。烟样经甲醇提取、XAD-2柱净化,得到初步纯化的糖苷,在pH 5条件下将其酶解,释放出糖苷配基。采用气相色谱-质谱分析并通过标准谱库检索确定了5种挥发性苷元;然后通过电喷雾质谱(负离子模式)确定糖苷母离子并作碎片离子扫描(MS2),确定了5种糖苷类香味前体物质的存在形式;最后采用UPLC-ESI MS/MS,以甲醇和乙酸-乙酸铵水溶液为流动相,通过RP-C18柱分离,在多反应监测(MRM)模式下,鉴定了烟草中5种主要的糖苷类香味前体物质,为应用液相色谱-质谱分析缺乏标准样品的糖苷类香味前体物质奠定了基础。

关键词: 超高效液相色谱-串联质谱, 气相色谱-质谱, 糖苷类香味前体物质, 烟草

Abstract: A qualitative method for the identification of 5 main glycosidic flavor precursors in tobacco was developed by using ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI MS/MS) and gas chromatography-mass spectrometry (GC-MS). The glycosidic flavor precursors in tobacco were extracted with methanol, cleaned up with an XAD-2 column. The aglycones were later released by enzyme-mediated hydrolysis under the condition of pH 5. The 5 volatile aglycone moieties were identified by GC-MS standard spectra library. The precursor ions of glycosides were determined by using electrospray ionization mass spectrometry in negative ion mode, then the 5 glycosidic flavor precursors were identified by using product ion scan (MS2) finally, using UPLC-ESI MS/MS, separation and identification of 5 glycosidic flavor precursors were accomplished on an RP-C18 column in the multiple reaction monitoring (MRM) mode by using methanol and acetic acid-ammonium acetate aqueous solution as eluent. This work lays a foundation for the analysis of glycosidic flavor precursors without the standards by using liquid chromatography-mass spectrometry.

Key words: gas chromatography-mass spectrometry (GC-MS), glycosidic flavor precursors, tobacco , ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)