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色谱

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非衍生化毛细管区带电泳直接紫外检测法测定茶叶中的4种氨基酸

王清萍1,2*, 张兰1, 陈国南1, 林金明3*   

  1. 1.Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China; 2.College of Chemistry and Materials, Fujian Normal University, Fuzhou 350007, China; 3.Department of Chemistry, Tsinghua University, Beijing 100084, China
  • 收稿日期:2009-08-14 修回日期:2009-10-14 出版日期:2009-11-30 发布日期:1981-12-25
  • 通讯作者: 王清萍，林金明

Determination of four amino acids in tea by capillary zone electrophoresis and direct ultraviolet detection without derivatization

WANG Qingping1,2*, ZHANG Lan1, CHEN Guonan1, LIN Jin-Ming3*   

  1. 1.Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China; 2.College of Chemistry and Materials, Fujian Normal University, Fuzhou 350007, China; 3.Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:2009-08-14 Revised:2009-10-14 Online:2009-11-30 Published:1981-12-25
  • Contact: WANG Qingping，LIN Jin-Ming

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摘要： 采用非衍生化毛细管区带电泳直接紫外检测法同时分离测定精氨酸(Arg)、色氨酸(Trp)、苯丙氨酸(Phe)和酪氨酸(Tyr)4种氨基酸,并应用于不同发酵过程的茶叶样品的测定。在分离电压为20 kV、柱温为25 ℃、检测波长为190 nm条件下,以25 mmol/L硼酸-硼砂缓冲溶液(pH 10.0)为运行缓冲液,4种组分在8 min内达到基线分离,Arg、Trp、Phe、Tyr的检出限分别为5.0,1.0,0.3和0.5 mg/L。7次平行测定中,4种组分迁移时间的相对标准偏差(RSD)均小于2.8%,峰电流的RSD均小于4.0%。将所建立的方法用于11种实际茶叶样品中Arg、Trp、Phe和Tyr含量的测定,结果令人满意。该方法可以为茶叶的质量评估提供借鉴。

关键词: 氨基酸, 茶叶 , 非衍生化, 毛细管区带电泳, 直接紫外检测

Abstract: Four important amino acids, arginine (Arg), tryptophane (Trp), phenylalanine (Phe) and tyrosine (Tyr) in tea samples with different fermentation processes were simultaneously determined by capillary zone electrophoresis (CZE) using direct ultraviolet detection (UV) at 190 nm. Under the conditions of 20 kV of separation voltage and 25 ℃ of temperature, Arg, Trp, Phe and Tyr were separated successfully within 8 min in 25 mmol/L sodium borate-boric acid (pH 10.0) with the detection limits of 5.0, 1.0, 0.3 and 0.5 mg/L for Arg, Trp, Phe and Tyr, respectively. The relative standard deviations (RSDs) of migration time (n=7) were all lower than 2.8% and the RSDs of peak electric current (n=7) were lower than 4.0%. The method was applied in the determination of Arg, Trp, Phe and Tyr in eleven tea samples. The results were satisfactory. This method can provide beneficial reference to evaluation quality of tea.

Key words: amino acid, capillary zone electrophoresis (CZE), direct ultraviolet detection, tea , underivatization