色谱

色谱

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Ti-SBA-15介孔材料用于磷酸化肽的高效富集

张宇, 秦洪强, 吴仁安, 邹汉法*   

  1. 中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 辽宁 大连 116023
  • 收稿日期:2009-09-10 修回日期:2009-11-10 出版日期:2010-02-28 发布日期:1981-06-25
  • 通讯作者: 邹汉法
  • 基金资助:
    国家自然科学基金项目No. 20735004

Applications of Ti-SBA-15 mesoporous material in high performance enrichment of phosphopeptides

ZHANG Yu, QIN Hongqiang, WU Ren’an, ZOU Hanfa*   

  1. Dalian Institute of Chemical Physics, Key Laboratory of Separation Science for Analytical Chemistry, Chinese Academy of Sciences, Dalian 116023, China
  • Received:2009-09-10 Revised:2009-11-10 Online:2010-02-28 Published:1981-06-25
  • Contact: ZOU Hanfa

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705

被引次数

19

摘要： 结合基质辅助激光解吸飞行时间质谱(MALDI-TOF MS)检测技术,考察了Ti-SBA-15介孔材料对β-酪蛋白酶解产物中磷酸化肽的选择性富集性能。实验结果显示,含Ti和Si物质的量比为0.08的Ti-SBA-15介孔材料可选择性地对β-酪蛋白酶解产物中的磷酸化肽进行选择性富集;对于β-酪蛋白和牛血清白蛋白物质的量比为1:100的蛋白质酶解混合液,Ti-SBA-15仍能实现对其磷酸化肽的有效富集。上述结果表明,作为一种多孔、高比表面积的磷酸化多肽的选择性吸附材料,Ti-SBA-15有望在磷酸化蛋白质组的分析中得到广泛的应用。

关键词: Ti-SBA-15介孔材料, 基质辅助激光解吸飞行时间质谱 , 磷酸化肽, 选择性富集

Abstract: A titanium-incorporated SBA-15mesoporous material (Ti-SBA-15) was synthesized via the co-condensation of tetraethyl orthosilicate (TEOS) and tetrabutyl titanate using surfactant P123 as the template. Due to the existence of Ti in the framework of SBA-15, the synthesized Ti-SBA-15 was applied as the selective adsorbent of phosphopeptides from the complex tryptic digest of β-casein. The matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) analysis showed that the phosphopeptides of β-casein digest could be selectively enriched by the Ti-SBA-15 with Ti/Si molar ratio of 0.08, even under the interference of bovine serum albumin (BSA) with the molar ratio of β-casein to BSA up to 1:100. It can be concluded that the Ti-SBA-15 showed the specific adsorption toward the phosphorylated peptides, which provides great potential in the specific capture of phosphopeptides.

Key words: matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) , phosphopeptides, selective enrichment, titanium-incorporated SBA-15 mesoporous material (Ti-SBA-15)