色谱

色谱

• 特别策划 • 上一篇    下一篇

色谱保留时间在蛋白质组研究中的应用

邵晨*, 高友鹤   

  1. 中国医学科学院基础医学研究所, 中国协和医科大学基础医学院, 生理和病理生理学系, 北京 100005
  • 收稿日期:2009-12-24 修回日期:2010-01-22 出版日期:2010-02-28 发布日期:1981-06-25
  • 通讯作者: 邵晨

Application of peptide retention time in proteome research

SHAO Chen*, GAO Youhe   

  1. Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing 100005, China
  • Received:2009-12-24 Revised:2010-01-22 Online:2010-02-28 Published:1981-06-25
  • Contact: SHAO Chen

PDF

812

被引次数

15

摘要： 液相色谱与串联质谱联用(LC-MS/MS)技术是蛋白质组学研究中的常见方法。保留时间作为独立于质谱信息的参数已经被用于蛋白质的鉴定和定量工作中。在多肽鉴定领域,多肽的色谱保留时间预测与常规的二级串联质谱数据库搜索算法结合可以提高鉴定的可信度。鉴定的灵敏度也可以通过匹配多次LC-MS实验中具有相同精确质量数和保留时间的峰而提高。另一方面,由于色谱条件的微小改变即会引起保留时间的变化,因此对多次实验结果进行保留时间比对是进行非标记定量的不可或缺的步骤。另外,联合保留时间偏移和质量数信息还可以进行蛋白质翻译后修饰(post-translational modification, PTM)的鉴定。

关键词: 保留时间比对, 保留时间预测, 蛋白质组学 , 多肽鉴定, 翻译后修饰, 非标记定量, 液相色谱-串联质谱

Abstract: Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has been one of the most popular approaches in proteome analysis. As an independent parameter to mass spectrometry information, peptide retention time has been utilized to facilitate protein identification and quantification. In the field of peptide identification, the prediction of the retention time combined with routine tandem mass spectrometry database searching methods could help improve the confidence of identification. The sensitivity of identification could also be improved by matching peaks with both the accurate mass and retention time in multiple aligned LC-MS runs. Meanwhile, because small changes of liquid chromatography conditions lead to variability in retention times unavoidably, retention time alignment is crucial to label-free quantification. Additionally, post-translational modifications (PTM) could be identified by combining retention time shifts and mass deviation information.

Key words: label-free quantification, peptide identification, post-translational modification (PTM), proteomics , retention time alignment, retention time prediction, liquid chromatography-tandem mass spectrometry (LC-MS/MS)