色谱

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涡流色谱技术在生物样品分析中的应用

刘朋, 周建良, 安婧婧, 李萍*   

  1. 现代中药教育部重点实验室, 中国药科大学生药学教研室, 江苏 南京 210009
  • 收稿日期:2009-08-28 修回日期:2009-10-28 出版日期:2010-02-28 发布日期:1981-06-25
  • 通讯作者: 李萍

Application of turbulent flow chromatography in the analysis of biological samples

LIU Peng, ZHOU Jianliang, AN Jingjing, LI Ping*   

  1. Key Laboratory of Modern Chinese Medicines, Ministry of Education, Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China
  • Received:2009-08-28 Revised:2009-10-28 Online:2010-02-28 Published:1981-06-25
  • Contact: LI Ping

摘要: 生物样品的复杂性使其在进行分析测定前必须经过处理。传统的样品前处理方法(如液-液萃取、固相萃取等)耗时长且操作繁琐。涡流色谱作为在线萃取技术,可以实现生物样品直接进样,减少了样品处理步骤,有效富集纯化了分析物,是一种高通量、高选择性的生物样品前处理方法。为此,本文介绍了涡流色谱技术的原理及优势,并总结了不同涡流柱的特点及其在生物样品分析领域中的应用情况。

关键词: 生物样品 , 涡流色谱, 样品前处理

Abstract: The complexity of biological fluids makes the sample preparation necessary in the analytical process. The traditional techniques, such as liquid-liquid extraction and solid-phase extraction, are time-consuming and labor-intensive. Turbulent flow chromatography (TFC), as an on-line direct injection technique, possesses the advantages of reducing sample preparation steps and enabling the effective pre-concentration and clean-up of biological fluids. Therefore, it is a high-throughput and high-selectivity technique for biological sample pretreatment. In this review, the theory, advantages, characteristics and current status of TFC in bioanalysis are introduced and summarized.

Key words: biological samples , sample pretreatment, turbulent flow chromatography (TFC)