色谱

色谱

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高效液相色谱双波长检测法测定维C银翘片中4种组分的含量

董丽*, 孙祥德, 李琴   

  1. 新乡医学院, 河南 新乡 453003
  • 收稿日期:2009-07-20 修回日期:2009-09-20 出版日期:2010-02-28 发布日期:1981-06-25
  • 通讯作者: 董丽
  • 基金资助:
    新乡医学院高学历人才基金项目No. 2005YJ31

Determination of four components in Vitamin C Yinqiao Tablets using reversed-phase high performance liquid chromatography with dual wavelength detection

DONG Li*, SUN Xiangde, LI Qin   

  1. Xinxiang Medical University, Xinxiang 453003, China
  • Received:2009-07-20 Revised:2009-09-20 Online:2010-02-28 Published:1981-06-25
  • Contact: DONG Li

PDF

777

被引次数

34

摘要： 建立了高效液相色谱测定维C银翘片中维生素C、对乙酰氨基酚、马来酸氯苯那敏和绿原酸4种组分含量的方法。研究优化了样品提取的溶剂与超声提取条件、色谱分离条件和检测波长等,采用了Sinochrom ODS-BP(4.6 mm×200 mm, 5 μm)色谱柱,以0.05 mol/L KH2PO4(pH 3.0,含1%三乙胺)-乙腈(75:25, v/v)为流动相,双检测波长260 nm和326 nm等分离检测条件。结果表明,维生素C、对乙酰氨基酚、马来酸氯苯那敏和绿原酸的测定线性范围均比较宽,方法平均回收率大于99.4%,相对标准偏差(RSD)小于1.8%(n=5),而且测定方法快速、简便、准确,非常适合维C银翘片类药物的质量控制。

关键词: 对乙酰氨基酚, 高效液相色谱法, 绿原酸, 马来酸氯苯那敏, 双检测波长, 维C银翘片 , 维生素C

Abstract: A reversed-phase high performance liquid chromatographic (RP-HPLC) method with dual wavelength detection was developed for the determination of vitamin C, paracetamol, chlorphenamine maleate and chlorogenic acid in Vitamin C Yinqiao Tablets. The separation was performed on a Sinochrom ODS-BP column with the mobile phase consisting of 0.05 mol/L KH2PO4 (pH 3.0, containing 1% triethylamine and acetonitrile (75:25, v/v). The detection wavelength was set at 260 nm (λ1) and 326 nm (λ2). The method linearities were wide and the average recoveries were more than 99.4%. The relative standard deviations were less than 1.8% (n=5). This method is convenient, rapid and accurate. It is readily applied to the quality controls in the production process of Vitamin C Yinqiao Tablets.

Key words: chlorogenic acid, chlorphenamine maleate, dual detection wavelengths, paracetamol, vitamin C, Vitamin C Yinqiao Tablets , high performance liquid chromatography (HPLC)