色谱

色谱

• 技术与应用 • 上一篇    下一篇

高效液相色谱手性固定相法拆分阿折地平对映体

张恺1, 薛娜2, 李林1, 李凡1, 杜玉民1*   

  1. 1. 河北医科大学药学院, 河北 石家庄 050017; 2. 河北化工医药职业技术学院, 河北 石家庄 050026
  • 收稿日期:2009-07-08 修回日期:2009-09-08 出版日期:2010-02-28 发布日期:1981-06-25
  • 通讯作者: 杜玉民

Enantiomeric separation of azelnidipine by high performance liquid chromatography with chiral stationary phase

ZHANG Kai1, XUE Na2, LI Lin1, LI Fan1, DU Yumin1*   

  1. 1. School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China; 2. Heibei Chemical and Pharmaceutical College, Shijiazhuang 050026, China
  • Received:2009-07-08 Revised:2009-09-08 Online:2010-02-28 Published:1981-06-25
  • Contact: DU Yumin

PDF

602

被引次数

21

摘要： 建立了阿折地平对映体的高效液相色谱拆分方法。采用Chiralpak AD-H (250 mm×4.6 mm, 5.0 μm, Daicel公司)手性色谱柱在正相条件下直接拆分阿折地平对映体,考察了固定相种类、流动相组成及柱温等对阿折地平对映体分离的影响。确定了最佳的拆分条件: 流动相为正己烷-异丙醇(90:10, v/v),流速为0.8 mL/min,检测波长为254 nm;柱温为20 ℃;在此条件下阿折地平对映体的分离度为3.3。该法简单快速,重现性好。

关键词: 阿折地平 , 高效液相色谱法, 手性拆分, 手性固定相

Abstract: A high performance liquid chromatographic (HPLC) method was established for the enantiomeric separation of azelnidipine. Baseline chiral separation of azelnidipine was achieved under normal-phase chromatographic mode using the Chiralpak AD-H chiral column (250 mm×4.6 mm, 5.0 μm, Daicel). The influences of the nature of chiral stationary phase, form of the mobile phase, and column temperature on the enantiomeric separation were studied. The optimized chromatographic conditions were hexane-isopropyl alcohol (90:10, v/v) as the mobile phase with a flow rate of 0.8 mL/min and detection at the wavelength of 254 nm. The column temperature was set at 20 ℃. The resolution of 3.3 for azelnidipine was achieved under the above chromatographic conditions. The method is simple, rapid and with good reproducibility.

Key words: azelnidipine , chiral split, chiral stationary phase, high performance liquid chromatography (HPLC)