色谱

色谱

• 研究论文 • 上一篇    下一篇

高效液相色谱-串联质谱法测定食品中20种氨基甲酸酯类农药残留

张帆1,2, 黄志强1*, 张莹1, 李忠海2, 王美玲1   

  1. 1. 湖南省检验检疫科学研究院, 湖南出入境检验检疫局技术中心, 食品安全科学技术湖南省重点实验室, 湖南 长沙 410004; 2. 中南林业科技大学食品科学与工程学院, 湖南 长沙 410004
  • 收稿日期:2009-12-08 修回日期:2010-02-08 出版日期:2010-04-28 发布日期:1980-12-25
  • 通讯作者: 黄志强

Determination of 20 carbamate pesticide residues in food by high performance liquid chromatography-tandem mass spectrometry

ZHANG Fan1,2, HUANG Zhiqiang1*, ZHANG Ying1, LI Zhonghai2, WANG Meiling1   

  1. 1. Hunan Academy of Inspection & Quarantine, Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau, Hunan Key Laboratory of Food Safety Science & Technology, Changsha 410004, China; 2. Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
  • Received:2009-12-08 Revised:2010-02-08 Online:2010-04-28 Published:1980-12-25
  • Contact: HUANG Zhiqiang

PDF

839

被引次数

120

摘要： 建立了食品中20种氨基甲酸酯类农药残留量的高效液相色谱-串联质谱联用(HPLC-MS/MS)测定与确证方法。20种氨基甲酸酯类农药在0.005～0.1 mg/kg范围内线性良好,相关系数为0.991 7～0.999 6;在0.005～0.025 mg/kg范围内, 20种目标物的回收率为51.2%～125.0%,相对标准偏差为1.4%～19.8%。该方法准确、灵敏、快速,可满足国际上对食品中这20种氨基甲酸酯类农药残留量的检测需要。

关键词: 氨基甲酸酯类农药, 残留分析, 高效液相色谱-串联质谱法, 食品

Abstract: A new method of determination and conformation for 20 carbamate pesticide residues in food by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The residual carbamate pesticides in food were extracted with acetonitrile, cleaned up with C18 solid phase extraction column or Carb/NH2 solid phase extraction column, and the targets were detected and confirmed by HPLC-MS/MS using external standard method. The recoveries of the 20 carbamate pesticides in spiked levels (from 0.005 to 0.025 mg/kg) ranged from 51.2% to 125.0% with the relative standard deviations (RSDs) from 1.4% to 19.8%; the concentration range of linearity was 0.005~0.1 mg/kg; the correlation coefficients were 0.991 7~0.999 6. The method is fast, easy, sensitive and reliable. It also demonstrates that this method can meet the requirements for the simultaneous determination of 20 carbamate pesticide residues in food.

Key words: carbamate pesticides, food , residues analysis, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)