色谱

色谱

• 技术与应用 • 上一篇    下一篇

自制炭阱吸附装置与气相色谱-质谱联用测定植物的挥发性有机物

郭淑政1,2, 刘苏静1, 马宣宣1,2, 杨翠云1, 周世伟1, 夏传海1*   

  1. 1. 中国科学院烟台海岸带研究所, 山东 烟台 264003; 2. 中国科学院研究生院, 北京 100049
  • 收稿日期:2010-03-08 修回日期:2010-05-08 出版日期:2010-07-28 发布日期:1980-03-25
  • 通讯作者: 夏传海

Determination of volatiles released from plants by the self-made charcoal trap adsorption device coupled with gas chromatography-mass spectrometry

GUO Shuzheng1,2, LIU Sujing1, MA Xuanxuan1,2, YANG Cuiyun1, ZHOU Shiwei1, XIA Chuanhai1*   

  1. 1. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2010-03-08 Revised:2010-05-08 Online:2010-07-28 Published:1980-03-25
  • Contact: XIA Chuanhai

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451

被引次数

4

摘要： 设计组装了炭阱吸附装置,并与气相色谱-质谱(GC-MS)联用测定了银杏叶和利马豆的挥发性有机物。采用炭阱吸附装置与固相微萃取(SPME)收集银杏叶的挥发性有机物,用GC-MS进行分析,结果表明采用炭阱吸附装置对银杏叶挥发性有机物的富集效果优于SPME方法。实验还采用内标法对利马豆的挥发性有机物进行了初步的定量,两次实验结果的重复性较好。由于炭阱吸附装置能够很好地收集植物的挥发性有机物,且可以进行多个样品的平行实验及植物挥发性有机物的定量研究,因此炭阱吸附装置与GC-MS联用更适合用于实验室中植物挥发性有机物的研究。

关键词: 挥发性有机物, 利马豆 , 气相色谱-质谱, 炭阱吸附装置, 银杏叶

Abstract: The charcoal trap adsorption device was designed and used to adsorb the volatile organic compounds (VOCs) emitted from Ginkgo leaves and Lima beans. The adsorbed VOCs were determined using gas chromatography-mass spectrometry (GC-MS). Compared to solid phase microextraction (SPME), the self-made charcoal trap adsorption device was more advantageous to the enrichment of VOCs from Ginkgo leaves. Additionally, the quantitative analysis of the collected VOCs released from Lima beans was carried out through the addition of internal standard, and the results of the two parallel experiments showed good repeatability. Because the eluent of the charcoal trap can be stored and used in the quantitative analysis as well as parallel experiments, the self-made charcoal trap adsorption device coupled with GC-MS is suitable for laboratory studies of plant volatile organic compounds.

Key words: gas chromatography-mass spectrometry (GC-MS), Ginkgo leaves, Lima beans , volatile organic compounds (VOCs), charcoal trap adsorption device