色谱

色谱 ›› 2010, Vol. 28 ›› Issue (08): 765-768.DOI: 10.3724/SP.J.1123.2010.00765

• 研究论文 • 上一篇    下一篇

基于液相色谱-质谱联用技术的代谢组学方法研究薄荷烟对大鼠代谢的影响

石先哲1*, 何智慧2, 窦阿波1, 张凤霞1, 练文柳2, 许国旺1   

  1. 1. 中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 辽宁 大连 116023; 2. 湖南中烟工业有限责任公司技术中心, 湖南 长沙 410007
  • 收稿日期:2010-05-12 修回日期:2010-06-23 出版日期:2010-08-28 发布日期:2010-08-28
  • 通讯作者: 石先哲,博士,副研究员,主要从事液相色谱-质谱联用及代谢组学研究.
  • 基金资助:

    国家自然科学基金项目(No. 20805045)和湖南中烟工业公司科技项目.

Effect of menthol cigarette on rats for metabonomics by liquid chromatography-mass spectrometry

SHI Xianzhe1*, HE Zhihui2, DOU Abo1, ZHANG Fengxia1, LIAN Wenliu2, XU Guowang1   

  1. 1. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. Research Center of China Tobacco Hunan Industrial Co., Ltd., Changsha 410007, China
  • Received:2010-05-12 Revised:2010-06-23 Online:2010-08-28 Published:2010-08-28

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被引次数

11

摘要： 将基于液相色谱-质谱联用(LC-MS)技术的代谢组学分析平台用于薄荷烟对大鼠代谢影响的研究。分析了3组大鼠的尿样,包括对照大鼠、吸食普通烟大鼠和吸食薄荷烟大鼠,并采用主成分分析(PCA)方法对数据进行模式识别。PCA得分图表明吸食薄荷烟大鼠与对照组大鼠尿样的代谢差异要小于吸食普通烟大鼠。从PCA载荷图中找到并鉴定了犬尿喹啉酸等8种重要代谢物。通过考察代谢物在对照大鼠、吸食薄荷烟大鼠和吸食普通烟大鼠尿样中的相对含量变化,进一步说明了烟草中添加薄荷醇可减少烟草对大鼠代谢的影响。

关键词: 薄荷烟, 大鼠, 代谢组学, 液相色谱-质谱联用

Abstract: A liquid chromatography-mass spectrometry (LC-MS) method was developed for the metabonomics study of menthol cigarette effect on rats. Urines from three groups of rats were analyzed, including control rats, rats treated with menthol cigarette and rats treated with normal cigarette, and the data were processed by the method of principal component analysis (PCA). The PCA score plot showed that the metabolic difference between the rats treated with menthol cigarette and the control rats was smaller than that between the rats treated with normal cigarette and the control rats. Based on the PCA score plot, eight important metabolites, for example, kynurenic acid, were found and identified. Their relative concentration changes among the three groups further indicated that menthol cigarette maybe decrease the metabolic effect on rats.

Key words: menthol cigarette, metabonomics, rats, liquid chromatography-mass spectrometry (LC-MS)