色谱

色谱 ›› 2010, Vol. 28 ›› Issue (09): 898-901.DOI: 10.3724/SP.J.1123.2010.00898

• 研究论文 • 上一篇    下一篇

离子色谱法测定婴幼儿配方奶粉中的核苷酸

叶明立*, 潘广文, 胡忠阳, 汪琼   

  1. 戴安中国有限公司应用研究中心上海实验室, 上海 201203
  • 收稿日期:2010-07-12 修回日期:2010-08-14 出版日期:2010-09-28 发布日期:2010-09-28
  • 通讯作者: 叶明立,硕士,主要从事食品分析工作.
  • 基金资助:

    浙江省自然科学基金项目(Nos. R4080124, Y507252)和浙江省分析测试基金项目(No. 2007F70061).

Determination of nucleotides in infant formula milk powder by ion chromatography

YE Mingli*, PAN Guangwen, HU Zhongyang, WANG Qiong   

  1. Shanghai Laboratory of Application and Research Center, Dionex China Limited, Shanghai 201203, China
  • Received:2010-07-12 Revised:2010-08-14 Online:2010-09-28 Published:2010-09-28

PDF

569

被引次数

27

摘要： 建立了婴幼儿配方奶粉中5种核苷酸的离子色谱检测方法。分离柱为IonPac AS16柱,以KOH为流动相,梯度洗脱,流速为1.0 mL/min,柱温为25 ℃,检测波长为260 nm,进样量为25 μL。在上述条件下,胞嘧啶核苷酸(CMP)、腺嘌呤核苷酸(AMP)、尿嘧啶核苷酸(UMP)、次黄嘌呤核苷酸(IMP)、鸟嘌呤核苷酸(GMP)的质量浓度分别为0.09～50、0.06～50、0.06～50、0.09～50、0.06～50 mg/L时与对应色谱峰面积之间的线性关系良好,检出限分别为0.03、0.02、0.02、0.03和0.02 mg/L,回收率为92.5%～104.0%。该方法简便、快速、准确,可用于实际样品的测定。

关键词: 核苷酸, 离子色谱法, 婴幼儿配方奶粉

Abstract: A method was developed for the determination of nucleotides in infant formula milk powder by ion chromatography (IC). The separation was performed on an IonPac AS16 column with KOH solution as the mobile phase at a flow rate of 1.0 mL/min and 25 ℃. The detection wavelength was set at 260 nm and the sample injection volume was 25 μL. There were good linear relationships between the mass concentrations and the peak areas of cytidine monophosphate (CMP), adenosine monophosphate (AMP), uridine monophosphate (UMP), inosine monophosphate (IMP) and guanosine monophosphate (GMP) in the ranges of 0.09~50, 0.06~50, 0.06~50, 0.09~50, 0.06~50 mg/L, respectively. The limits of detection (S/N=3) of CMP, AMP, UMP, IMP and GMP were 0.03, 0.02, 0.02, 0.03 and 0.02 mg/L, respectively. The method has been applied for the determination of the five nucleotides in infant formula milk powder with the recoveries of 92.5%~102.4%. This method is rapid, simple and suitable for the determination of real samples.

Key words: infant formula milk powder, nucleotides, ion chromatography (IC)