色谱

色谱 ›› 2010, Vol. 28 ›› Issue (10): 915-922.DOI: 10.3724/SP.J.1123.2010.00915

• 专论与综述 • 上一篇    下一篇

高效硅胶化学键合固定相的研究进展

佟巍1,2, 张养军1, 秦伟捷1, 钱小红1*   

  1. 1. 蛋白质组学国家重点实验室, 军事医学科学院放射与辐射医学研究所, 北京蛋白质组研究中心, 北京 102206; 2. 中国人民武装警察部队医学院药学系, 天津 300162
  • 收稿日期:2010-07-13 修回日期:2010-08-30 出版日期:2010-10-28 发布日期:2010-10-28
  • 通讯作者: 钱小红,研究员,研究方向为蛋白质化学与蛋白质组学.
  • 基金资助:

    国家重点基础研究规划项目(2006CB910803, 2007CB914104)、国家高技术研究发展计划项目(2006AA02A308, 2008AA02Z309)、国家自然科学基金项目(30621063, 20635010, 20735005, 20875101)、蛋白质组学国家重点实验室项目(SKLP-K200807, SKLP-O200808).

Recent advances of high performance chemically bonded silica gel stationary phases

TONG Wei1,2, ZHANG Yangjun1, QIN Weijie1, QIAN Xiaohong1*   

  1. 1. State Key Laboratory of Proteomics, Beijing Institute of Radiation Medicine, Beijing Proteome Research Center, Beijing 102206, China; 2. Department of Pharmacy, Medical College of Chinese People’s Armed Police Forces, Tianjin 300162, China
  • Received:2010-07-13 Revised:2010-08-30 Online:2010-10-28 Published:2010-10-28

PDF

2951

被引次数

18

摘要： 对高效液相色谱用硅胶作为基质的化学键合固定相的研究进展进行了全面的评述。介绍了硅胶基质填料的物理化学性质及前处理过程,详细阐述了化学键合固定相的键合反应机制和种类,概括了化学键合固定相在高效液相色谱中的应用,并对我国的硅胶基质填料研究和应用前景进行了展望。

关键词: 高效液相色谱, 硅胶基质填料, 化学键合固定相, 进展

Abstract: The advances of the development and the application of silica packing medium in high performance liquid chromatography (HPLC) are reviewed. The physicochemical properties on surface and the methods of pre-processing of this medium are introduced. The bonding modes and the classes of chemically bonded stationary phase are described. The applications of silica packing medium in HPLC and its developing trends are also outlined.

Key words: advances, chemically bonded stationary phase, high performance liquid chromatography (HPLC), silica packing medium