色谱

色谱 ›› 2010, Vol. 28 ›› Issue (10): 984-988.DOI: 10.3724/SP.J.1123.2010.00984

• 研究论文 • 上一篇    下一篇

高效阴离子交换色谱-脉冲安培法检测小鼠尿液中的甘露醇、单糖和乳果糖

周利1, 刘菊2, 郑婷2, 丁卉2, 施超欧2*   

  1. 1. 华东理工大学 生物反应器工程国家重点实验室, 上海 200237; 2. 华东理工大学分析测试中心, 上海 200237
  • 收稿日期:2010-06-22 修回日期:2010-07-23 出版日期:2010-10-28 发布日期:2010-10-28
  • 通讯作者: 施超欧,高级工程师,主要研究方向为离子色谱及液相色谱的应用研究. Tel: (021)64252812, E-mail: hplc@ecust.edu.cn.
  • 基金资助:

    无

Determination of mannitol, monosaccharides and lactulose in mouse urine by high performance anion exchange chromatography coupled with pulsed amperometric detection

ZHOU Li1, LIU Ju2, ZHENG Ting2, DING Hui2, SHI Chaoou2*   

  1. 1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2. Analysis and Research Center, East China University of Science and Technology, Shanghai 200237, China
  • Received:2010-06-22 Revised:2010-07-23 Online:2010-10-28 Published:2010-10-28

PDF

680

被引次数

8

摘要： 采用高效阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)建立了小鼠尿液中甘露醇、单糖(包括半乳糖、葡萄糖、甘露糖和果糖)和乳果糖的分析方法。样品经离心沉淀除去蛋白并过分子膜,以CarboPacTM PA1阴离子交换柱为分离柱,采用NaOH梯度淋洗,脉冲安培四电位检测。结果表明,甘露醇、半乳糖、葡萄糖、甘露糖、果糖和乳果糖在0.1～5.0 mg/L内线性良好,线性相关系数r2为0.988～0.999,样品加标回收率为95.5%～104.2%,检出限为0.0013～0.0048 mg/L。此法准确、快速、简便,能同时对6种糖类化合物进行分析,可以跟踪检测整个糖类代谢过程中甘露醇、单糖和乳果糖之间的代谢关系。

关键词: 单糖, 甘露醇, 高效阴离子交换色谱, 脉冲安培检测法, 乳果糖

Abstract: A method of high performance anion exchange chromatography (HPAEC) coupled with pulsed amperometric detection (PAD) was established for the determination of mannitol, monosaccharides (galactose, glucose, mannose and fructose) and lactulose in mouse urine. The samples were first centrifuged, and then filtered through molecular film to eliminate the proteins. The analysis was performed on a CarboPacTM PA1 column using gradient elution with water and 250 mmol/L sodium hydroxide as the mobile phase. All of the six carbohydrates had good linear relationships (0.988≤r2≤0.999) in the range of 0.1~5.0 mg/L. The recoveries were between 95.5% and 104.2% and the limits of detection were between 0.0013 and 0.004 8 mg/L. The method is accurate, fast and simple. It can be used to analyse six carbohydrates simultaneously and track the metabolic relationships among mannitol, monosaccharides and lactulose during the metabolism of the carbohydrates.

Key words: lactulose, mannitol, monosaccharide, pulsed amperometric detection, high performance anion exchange chromatography