色谱

色谱 ›› 2010, Vol. 28 ›› Issue (11): 1067-1070.DOI: 10.3724/SP.J.1123.2010.01067

• 研究论文 • 上一篇    下一篇

高效液相色谱指纹图谱法分析南方红豆杉药材的氯仿提取物

李效贤, 熊耀康*, 余陈欢, 张春椿   

  1. 浙江中医药大学药学院, 浙江 杭州 310053
  • 收稿日期:2010-05-31 修回日期:2010-07-18 出版日期:2010-11-28 发布日期:2010-11-28
  • 通讯作者: 熊耀康,教授,博士生导师,主要从事中药资源开发与利用.
  • 基金资助:

    浙江省中医药管理重大项目(2007ZA002)、浙江省科技厅项目(2008C32020)和浙江省中药现代化项目(浙财企字［2009］331).

High performance liquid chromatographic fingerprints method for the analysis of chloroform extracts of Taxus wallichiana

LI Xiaoxian, XIONG Yaokang*, YU Chenhuan, ZHANG Chunchun   

  1. School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China
  • Received:2010-05-31 Revised:2010-07-18 Online:2010-11-28 Published:2010-11-28

PDF

558

被引次数

13

摘要： 建立了南方红豆杉药材氯仿提取物的高效液相色谱(HPLC)指纹图谱分析方法。采用Eurospher 100 C18色谱柱(250 mm×4 mm, 5 μm),以甲醇和水为流动相进行梯度洗脱,流速为1 mL/min,检测波长为232 nm,柱温为30 ℃。以10-脱乙酰巴卡亭III(10-DABIII)为参照物,在相同的色谱条件下测定了10批不同产地的南方红豆杉药材氯仿提取物的指纹图谱,获得了11个共有指纹峰,并利用主成分分析法(PCA)对指纹图谱进行统计分析。结果表明南方红豆杉药材的质量与种植区域有关。该方法稳定、可靠,可用于南方红豆杉药材的质量控制。

关键词: 高效液相色谱法, 南方红豆杉, 指纹图谱, 主成分分析

Abstract: A high performance liquid chromatographic (HPLC) fingerprints method was developed for the analysis of the chloroform extracts of Taxus wallichiana. The extracts were separated on an Eurospher 100 C18 column (250 mm×4 mm, 5 μm) with the gradient elution of methanol and water at a flow rate of 1 mL/min. The detection wavelength was set at 232 nm, and the column temperature was 30 ℃. Under the same conditions, 10 batches of the chloroform extracts of Taxus wallichiana from different habitats were analyzed with the reference substance of 10-deacetyl baccatin III (10-DABIII). The 11 peaks were selected as the characteristic peaks, and the method of principal component analysis (PCA) was applied to analyze the fingerprints of the chloroform extracts of Taxus wallichiana. The results showed that there was an interrelated relationship between habitat and quality of Taxus wallichiana. The method has good repeatability and stability, and can be utilized as an approach for the quality control of Taxus wallichiana.

Key words: fingerprint, principal component analysis (PCA), Taxus wallichiana, high performance liquid chromatography (HPLC)