色谱

色谱 ›› 2010, Vol. 28 ›› Issue (11): 1020-1025.DOI: 10.3724/SP.J.1123.2010.01020

• 研究论文 • 上一篇    下一篇

高效液相色谱-串联质谱法测定食品中6种人工合成甜味剂

刘晓霞1,2, 丁利1, 刘锦霞2, 张莹1*, 黄志强1, 王利兵1, 陈波2   

  1. 1. 湖南省检验检疫科学技术研究院, 湖南出入境检验检疫局技术中心, 食品安全科学技术湖南省重点实验室, 湖南 长沙 410004; 2. 湖南师范大学化学生物学及中药分析省部共建教育部重点实验室, 湖南 长沙 410081
  • 收稿日期:2010-07-21 修回日期:2010-09-28 出版日期:2010-11-28 发布日期:2010-11-28
  • 通讯作者: 张莹,博士,研究员,研究方向为食品安全检测. Tel: (0731)85627802.
  • 基金资助:

    湖南省重大科技专项湖南省食品安全监控技术体系研究与示范课题(2009FJ1017).

Simultaneous determination of six synthetic sweeteners in food by high performance liquid chromatography-tandem mass spectrometry

LIU Xiaoxia1,2, DING Li1, LIU Jinxia2, ZHANG Ying1*, HUANG Zhiqiang1, WANG Libing1, CHEN Bo2   

  1. 1. Hunan Academy of Inspection and Quarantine, Technical Center of Hunan Entry-Exit Inspection and Quarantine Bureau, Hunan Key Laboratory of Food Safety Science & Technology, Changsha 410004, China; 2. Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China
  • Received:2010-07-21 Revised:2010-09-28 Online:2010-11-28 Published:2010-11-28

PDF

910

被引次数

65

摘要： 建立了食品中6种人工合成甜味剂(甜蜜素、糖精钠、安赛蜜、阿斯巴甜、阿力甜、纽甜)的高效液相色谱-串联质谱检测方法。样品经甲醇-水溶液(1:1, v/v)提取,以C18柱为分离柱,0.1%(v/v)甲酸-5 mmol/L甲酸铵溶液/乙腈为流动相,经高效液相色谱分离,采用电喷雾串联四极杆质谱进行检测。结果表明,6种人工合成甜味剂在20～500 μg/L范围内定量离子对的响应峰面积和样品质量浓度之间有良好的线性关系(相关系数＞0.998)。在3个添加水平下,样品平均回收率为81.3%～106.0%,相对标准偏差小于11%。该方法简单、灵敏、准确,可用于食品中6种人工合成甜味剂的同时检测。

关键词: 阿力甜, 阿斯巴甜, 安赛蜜, 高效液相色谱-串联质谱, 纽甜, 食品, 糖精钠, 甜蜜素

Abstract: A simple and sensitive method for the determination of six synthetic sweeteners (sodium cyclamate, saccharin sodium, acesulfame-K, aspartame, alitame and neotame) in food was developed. The synthetic sweeteners were extracted by methanol-water (1:1, v/v). The extract was separated on a C18 column using 0.1% (v/v) formic acid-5 mmol/L ammonium formate/acetonitrile as mobile phase, and then detected by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) using multiple reaction monitoring (MRM) mode. The good linearities (r＞0.998) were achieved for all the analytes over the range of 20~500 μg/L. The recoveries obtained ranged from 81.3% to 106.0% at three spiked concentrations, with the relative standard deviations lower than 11%. The established method has been successfully applied to the determination of synthetic sweeteners in food.

Key words: acesulfame-K, alitame, aspartame, food, neotame, saccharin sodium, sodium cyclamate, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)