色谱

色谱 ›› 2010, Vol. 28 ›› Issue (11): 1089-1093.DOI: 10.3724/SP.J.1123.2010.01089

• 研究论文 • 上一篇    下一篇

毛细管区带电泳法拆分匹伐他汀钙对映体

程晓昆1,2, 王利娟1,2, 杨更亮1,2*, 程佳1,2, 张轶华3   

  1. 1. 河北大学药学院, 河北 保定 071002; 2. 河北省药物质量分析控制重点实验室, 河北 保定 071002; 3. 河北省药品检验所, 河北 石家庄 050011
  • 收稿日期:2010-07-26 修回日期:2010-08-30 出版日期:2010-11-28 发布日期:2010-11-28
  • 通讯作者: 杨更亮,教授,博士生导师.
  • 基金资助:

    国家自然科学基金项目(No. 20675084)、河北省科技攻关项目(Nos. 06276479D, 07276407D)和河北省教育厅项目(No. Z2007225).

Chiral separation of pitavastatin calcium enantiomers by capillary zone electrophoresis

CHENG Xiaokun1,2, WANG Lijuan1,2, YANG Gengliang1,2*, CHENG Jia1,2, ZHANG Yihua3   

  1. 1. College of Pharmacy, Hebei University, Baoding 071002, China; 2. Hebei Province Key Laboratory of Pharmaceutical Quality Control, Baoding 071002, China; 3. Hebei Provincial Institute for Drug Control, Shijiazhuang 050011, China
  • Received:2010-07-26 Revised:2010-08-30 Online:2010-11-28 Published:2010-11-28

PDF

587

摘要： 建立了匹伐他汀钙对映体的毛细管区带电泳(CZE)拆分方法。分别考察了电泳电压,缓冲溶液种类、浓度及pH值,环糊精种类及浓度,添加剂种类及浓度等参数对实验结果的影响,从而确定了匹伐他汀钙对映体的最佳拆分条件: 电泳电压为18 kV;运行缓冲溶液为80 mmol/L的Tris-HCl缓冲体系,pH值为3.20,其中含有50 mmol/L HP-β-CD(羟丙基-β-环糊精)和5 mmol/L SDS(十二烷基磺酸钠);采用重力进样,进样高度17 cm,进样时间为2 s。在优化的实验条件下,匹伐他汀钙对映体得到了较好的分离,分离度可达2.17。实验结果表明该方法可用于匹伐他汀钙对映体的分离,具有快速、便捷、准确性好等优点。

关键词: 对映体, 毛细管区带电泳, 匹伐他汀钙, 手性拆分

Abstract: A method of capillary zone electrophoresis (CZE) has been established for the chiral separation of pitavastatin calcium enantiomers. Several parameters, such as the running voltage, the composition and the pH value of the running buffer, the types of additive and so on, were evaluated. The optimal separation conditions were as follows: capillary, 53 cm (45 cm effective length, 50 μm); running buffer, 80 mmol/L Tris-HCl, pH 3.20, which contained 50 mmol/L HP-β-CD (hydroxypropyl-β-cyclodextrin) and 5 mmol/L SDS (sodium dodecylsulphate); applied voltage, 18 kV (54 μA); column temperature, 23 ℃; injection, 2 s at the height of 17 cm. Under these conditions, pitavatatin calcium enantiomers were separated well with the resolution of 2.17. The results indicate that this method is rapid, simple, accurate and suitable for the chiral separation of pitvatin calcium enantiomers.

Key words: chiral separation, enantiomers, pitavastatin calcium, capillary zone electrophoresis (CZE)