色谱

色谱 ›› 2010, Vol. 28 ›› Issue (11): 1061-1066.DOI: 10.3724/SP.J.1123.2010.01061

• 研究论文 • 上一篇    下一篇

高效液相色谱法测定化妆品中的泛酸及D-泛醇

毛希琴*, 胡侠, 潘炜   

  1. 大连市产品质量监督检验所, 辽宁 大连 116023
  • 收稿日期:2010-09-03 修回日期:2010-10-12 出版日期:2010-11-28 发布日期:2010-11-28
  • 通讯作者: 毛希琴,博士,高级工程师,主要研究方向为食品、药品、包装材料以及化妆品中功效成分及禁限用成分检测方法的研究与开发.
  • 基金资助:

    国家标准计划项目(No. 20081995-T-607).

Simultaneous determination of pantothenic acid and D-panthenol in cosmetics by high performance liquid chromatography

MAO Xiqin*, HU Xia, PAN Ｗei   

  1. Dalian Institute of Product Quality Supervision & Inspection, Dalian 116023, China
  • Received:2010-09-03 Revised:2010-10-12 Online:2010-11-28 Published:2010-11-28
  • Contact: Xi-qin Mao

PDF

887

被引次数

10

摘要： 建立了对不同基质化妆品(膏霜、乳液、水剂化妆品、油剂化妆品、蜡基化妆品、指甲油等)中泛酸(维生素B5)及D-泛醇(维生素原B5)的富集方法及同时测定的高效液相色谱(HPLC)法。利用水和与水不互溶的有机溶剂组成的双液相体系,首先将泛酸及D-泛醇与化妆品中油溶性成分及表面活性剂等基质成分初步分离,然后用亚铁氰化钾-乙酸锌共沉淀剂去除提取液中的水溶性成分,继而在酸性条件下将泛酸和D-泛醇富集于C18固相萃取填料上,脱除其他水溶性干扰物后,用40%甲醇水溶液洗脱,用HPLC分离,紫外检测器检测,外标法定量。该方法在泛酸和D-泛醇的含量为0.1～10 μg/g范围内有很好的线性,线性相关系数分别为0.9989和0.9996。不同化妆品基质中目标成分的方法回收率均在90%以上。对泛酸及D-泛醇的检出限均为30 μg/g,定量限均为100 μg/g。实验表明该方法可用于化妆品中泛酸及D-泛醇的同时测定,结果准确可靠。

关键词: D-泛醇, 泛酸, 高效液相色谱法, 化妆品

Abstract: A high performance liquid chromatographic method (HPLC) and sample pretreatment method were developed for the simultaneous determination of pantothenic acid (vitamin B5) and D-panthenol (provitamin B5) in cosmetics with different matrices (including of creams, lotions, aqueous cosmetics, oily cosmetics, wax-based cosmetics, nail polish etc). A liquid-liquid extraction system composed of water and water-immiscible solvent was used to preliminarily separate the target components from other oil-soluble components and surfactants in cosmetics, then macromolecular water-soluble matrices in cosmetics were removed by coprecipitation with potassium ferrocyanide-zinc acetate precipitating agent, and then under acid condition, pantothenic acid and D-panthenol were enriched on a C18 solid-phase extraction sorbent. After the removal of other water-soluble impurities, target components were eluted by 40% methanol and then separated and quantitatively analyzed by high performance liquid chromatography with external standard method. Good linear relationship was achieved in the concentration range of 0.1~10 μg/g for pantothenic acid and D-panthenol. The linear correlation coefficients were separately 0.9989 and 0.9996. The average recoveries of the target components in cosmetics were more than 90%. Limit of detection of the method was 30 μg/g and the limit of quantification was 100 μg/g. This method can be used to simultaneously determine pantothenic acid and D-panthenol in cosmetics. The results are accurate and reliable.

Key words: cosmetic, D-panthenol, high performance liquid chromatography (HPLC), pantothenic acid