色谱

色谱 ›› 2010, Vol. 28 ›› Issue (11): 1107-1110.DOI: 10.3724/SP.J.1123.2010.01107

• 技术与应用 • 上一篇    下一篇

分子印迹固相萃取-高效毛细管电泳法检测鸡肉中的恩诺沙星残留

汪雪雁1, 檀华蓉2, 祁克宗3*, 邵黎3, 李慧3, 薛秀恒1, 谢英1   

  1. 1. 安徽农业大学茶与食品科技学院, 安徽 合肥 230036; 2. 安徽农业大学生物技术中心, 安徽 合肥 230036; 3. 安徽农业大学动物科技学院, 安徽 合肥 230036
  • 收稿日期:2010-07-14 修回日期:2010-08-16 出版日期:2010-11-28 发布日期:2010-11-28
  • 通讯作者: 祁克宗,博士,教授,主要从事动物疫病病理研究.
  • 基金资助:

    国家“十一五”科技支撑项目子项目(2006BAK02A08-4).

Determination of enrofloxacin residue in chicken muscle using molecular imprinted solid phase extraction-high performance capillary electrophoresis

WANG Xueyan1, TAN Huarong2, QI Kezong3*, SHAO Li3, LI Hui3, XUE Xiuheng1, XIE Ying1   

  1. 1. College of Tea ＆ Food Science and Technology, Anhui Agricultural University, Hefei 230036, China; 2. Biotechnology Center, Anhui Agricultural University, Hefei 230036, China; 3. College of Animal Science, Anhui Agricultural University, Hefei 230036, China
  • Received:2010-07-14 Revised:2010-08-16 Online:2010-11-28 Published:2010-11-28

PDF

655

被引次数

68

摘要： 以恩诺沙星为模板分子,α-甲基丙烯酸为功能单体,二甲基丙烯酸乙二醇酯为交联剂,制备了恩诺沙星分子印迹聚合物。以该分子印迹聚合物为固相萃取材料,采用高效毛细管电泳分离方式,建立了分子印迹固相萃取-高效毛细管电泳检测鸡肉中恩诺沙星的方法。结果表明,该方法能有效地萃取和检测鸡肉中的恩诺沙星。在优化条件下,恩诺沙星的检出限为92.02 μg/kg,定量限为336.04 μg/kg;不同恩诺沙星添加水平下的回收率为77.84%～86.52%,相对标准偏差为2.18%～3.76%。该方法适用于鸡肉中恩诺沙星残留的测定。

关键词: 恩诺沙星, 分子印迹聚合物, 高效毛细管电泳, 固相萃取, 鸡肉

Abstract: Molecular imprinted polymer targeted for enrofloxacin (ENR) was synthesized using ENR as the template molecules, α-methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linking agent. With the ENR molecular imprinted polymer, a molecular imprinted solid phase extraction-high performance capillary electrophoresis (MISPE-HPCE) method for the determination of ENR in chicken muscle was developed. The results showed that this method exhibited high selectivity and sensitivity for the determination of ENR in chicken muscle. Under the optimized conditions, the limit of detection and limit of quantification were 92.02 μg/kg and 336.04 μg/kg, respectively. The recoveries of the ENR spiked in chicken muscle at different levels were in the range of 77.84%~86.52%, and the relative standard deviations (RSDs) were in the range of 2.18%~3.76%. This MISPE-HPCE method is feasible for the analysis of ENR residue in chicken muscle.

Key words: chicken muscle, enrofloxacin, high performance capillary electrophoresis (HPCE), solid phase extraction (SPE), molecular imprinted polymer (MIP)