色谱

色谱 ›› 2010, Vol. 28 ›› Issue (12): 1117-1122.DOI: 10.3724/SP.J.1123.2010.01117

• 专论与综述 • 上一篇    下一篇

二维液相色谱接口技术

丁坤1,2, 吴大朋1, 关亚风1*   

  1. 1. 中国科学院大连化学物理研究所仪器分析化学研究室, 中国科学院分离分析化学重点实验室, 辽宁 大连 116023; 2. 中国科学院研究生院, 北京 100049
  • 收稿日期:2010-09-25 修回日期:2010-10-25 出版日期:2010-12-28 发布日期:2010-08-28
  • 通讯作者: 关亚风,博士,研究员,主要研究方向为样品前处理与色谱分离、检测器及现场传感器. Tel: (0411)84379590, E-mail: guanyafeng@dicp.ac.cn.
  • 基金资助:

    国家自然科学基金项目(No. 20635010和No. 90917020)和安捷伦公司大学研究基金项目(No. 08CHN-537UR).

Interface of two dimensional liquid chromatography

DING Kun1,2, WU Dapeng1, GUAN Yafeng1*   

  1. 1. Department of Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, Key Lab of Separation Science for Analytical Chemistry, Chinese Academy of Sciences, Dalian 116023, China; 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2010-09-25 Revised:2010-10-25 Online:2010-12-28 Published:2010-08-28

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摘要： 二维液相色谱具有峰容量大、分辨率高、分析速度快等优点,已经成为复杂样品分离分析的重要工具。两种分离模式的转换通常需要经过一个特殊接口来完成,接口是二维液相色谱系统的核心,也是限制二维液相色谱应用的瓶颈;两种流动相不互溶时,接口尤为重要。本文针对二维液相色谱接口技术近期的发展和应用进行总结。引用文献51篇。

关键词: 二维液相色谱, 复杂样品, 接口

Abstract: Two dimensional liquid chromatography (2D-LC) has become an important analytical tool for the separation of complex samples due to enhanced peak capacity and selectivity compared to one-dimensional LC. The interface of the two dimensions is the key technology in 2D-LC system. The interface shall provide mobile phase removal from the 1st D-LC and solutes focusing before the fraction transfer into the 2nd D-LC. A comprehensive review on the interface is presented, and 51 references are cited.

Key words: complex samples, interface, two dimensional liquid chromatography (2D-LC)