色谱

色谱 ›› 2010, Vol. 28 ›› Issue (12): 1173-1178.DOI: 10.3724/SP.J.1123.2010.01173

• 研究论文 • 上一篇    下一篇

一种聚甲基丙烯酸酯类亲水作用毛细管电色谱整体柱的制备与评价

黄桂华1, 陈思谨2, 林旭聪2*, 谢增鸿2   

  1. 1. 福建中医药大学药学院, 福建 福州 350108; 2. 福州大学食品安全与环境监测技术研究所, 福建 福州 350108
  • 收稿日期:2010-09-03 修回日期:2010-10-09 出版日期:2010-12-28 发布日期:2010-08-28
  • 通讯作者: 林旭聪,博士,副教授,研究方向为现代色谱分离. Tel: (0591)22866131, E-mail: xulin@fzu.edu.cn.
  • 基金资助:

    福建省自然科学基金项目(No. 2009J05072).

Preparation and evaluation of a polymethacrylate based monolithic column for hydrophilic interaction capillary electrochromatography

HUANG Guihua1, CHEN Sijin2, LIN Xucong2*, XIE Zenghong2   

  1. 1. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China; 2. Institute of Food Safety and Environmental Monitoring, Fuzhou University, Fuzhou 350108, China
  • Received:2010-09-03 Revised:2010-10-09 Online:2010-12-28 Published:2010-08-28

PDF

725

被引次数

14

摘要： 以2-羟基乙基甲基丙烯酸酯(HEMA)为单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,制备了亲水分离模式的聚HEMA-co-EDMA新型毛细管电色谱(CEC)整体柱。考察了整体柱的结构特征及其CEC性能,研究了极性物质的保留行为,并对其可能的保留机理进行了探讨。所制备的整体柱稳定性好,固定相表面带有极性羟基功能团,不仅能提供亲水相互作用位点,且能吸附流动相中的阴离子产生阳极电渗流(EOF)。在流动相中乙腈含量较高(＞62%,体积分数)的条件下,整体柱表现出典型的亲水作用,实现了对核苷、碱基和苯胺类带有碱性的强极性化合物的高效分离,并成功分离了苯酚类、苯甲酸类等中性或酸性的极性化合物。

关键词: 聚合整体柱, 毛细管电色谱, 亲水作用

Abstract: A hydrophilic monolithic column was prepared by in-situ polymerization of 2-hydroxyethyl methacrylate (HEMA) as a functional monomer and ethylene glycol dimethacrylate (EDMA) as a cross-linking agent in the fused-silica capillary. The monolithic column was evaluated as a hydrophilic interaction capillary electrochromatography (HI-CEC) stationary phase for polar solutes. The polar sites on the surface of the monolithic stationary phase responsible for hydrophilic interactions were provided from the hydroxy groups on the surface of the monolithic stationary phase. These polar functionalities also attract ions from the mobile phase and impart the monolithic stationary phase with a given zeta potential to generate electro-osmotic flow (EOF). The characterization and the performance of the poly(HEMA-co-EDMA) monolithic column were carried out. The electrochromatographic retention behaviors of polar solutes were investigated and the retention mechanism was also studied. Hydrophilic interaction was observed at high acetonitrile content (＞62%, v/v). The results also show that the monolithic column can effectively separate polar solutes including phenols, benzoic acids, anilines, nucleosides and nucleic acid bases under HI-CEC mode.

Key words: hydrophilic interaction, polymer-based monolithic column, capillary electrochromatography (CEC)