色谱

色谱 ›› 2010, Vol. 28 ›› Issue (12): 1150-1153.DOI: 10.3724/SP.J.1123.2010.01150

• 研究论文 • 上一篇    下一篇

反相高效液相色谱法测定药用植物大红袍中的两个生物活性成分

谭青1, 寿清耀1, 张盛1, 沈征武1,2*   

  1. 1. 上海中医药大学中药学院, 上海 201203; 2. 巴塞利亚药业(中国)有限公司, 江苏 海门 226100
  • 收稿日期:2010-09-06 修回日期:2010-10-20 出版日期:2010-12-28 发布日期:2010-08-28
  • 通讯作者: 沈征武,研究员,博士生导师,研究方向为天然产物分离鉴定与合成. Tel: (0513)82198003, E-mail: jeff_shen_1999@yahoo.com.
  • 基金资助:

    国家人事部2007年留学回国人员优秀项目资助.

Determination of two bio-active compounds in Campylotropis hirtella (Franch.) Schindl. using reversed-phase high performance liquid chromatography

TAN Qing1, SHOU Qingyao1, ZHANG Sheng1, SHEN Zhengwu1,2*   

  1. 1. School of Chinese Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2. Basilea Pharmaceutica China Ltd., Haimen 226100, China
  • Received:2010-09-06 Revised:2010-10-20 Online:2010-12-28 Published:2010-08-28
  • Contact: zheng-wu SHEN

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523

被引次数

5

摘要： 建立了反相高效液相色谱-二极管阵列检测器(RP-HPLC-DAD)测定药用植物大红袍中具有抗菌活性的异黄酮类化合物3′-geranyl-5,7,4′-trihydroxyisoflavone(化合物1)及具有良好免疫抑制活性的紫檀烯类化合物8,9-dihydroxy-1-methoxy-［6′,6′-dimethylpyrano(2′,3′: 2,3)］pterocarpene(化合物2)含量的方法。采用的色谱柱为Agilent Zorbax SB-C18柱(250 mm×4.6 mm, 5 μm),以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,流速为1.0 mL/min;柱温30 ℃。化合物1和化合物2分别在4.4～13.2 μg和0.428～1.284 μg范围内呈线性关系;平均回收率分别为99.65%和99.11%,相对标准偏差分别为1.83%和2.59%(n=5)。该方法快速简便,灵敏度和分离度好,适用于大红袍药材中活性黄酮类成分的测定。

关键词: 大红袍, 反相高效液相色谱法, 药用植物, 异黄酮类化合物, 紫檀烯类化合物

Abstract: A method of reversed-phase high performance liquid chromatography (RP-HPLC) using diode array detection (DAD) was developed for the quantitative determination of 3′-geranyl-5,7,4′-trihydroxyisoflavone (compound 1) and 8,9-dihydroxy-1-methoxy-［6′,6′-dimethylpyrano(2′,3′: 2,3)］pterocarpene (compound 2) in Campylotropis hirtella. The separation and quantification were achieved using an Agilent Zorbax SB-C18 column (250 mm×4.6 mm, 5 μm), and mobile phases of acetonitrile and 0.1% formic acid with gradient elution at a flow rate of 1.0 mL/min and 30 ℃. The calibration curves for compounds 1 and 2 were linear in the ranges of 4.4~13.2 μg and 0.428~1.284 μg, respectively. The recoveries were 99.65% and 99.11% with the relative standard deviations of 1.83% and 2.59% (n=5), respectively. This RP-HPLC-DAD method is rather simple, accurate and convenient. It can be used for the quantitative determination of the active flavonoids in Campylotropis hirtella (Franch.) Schindl.

Key words: Campylotropis hirtella (Franch.) Schindl., isoflavone, medicinal plant, pterocarpene, reversed-phase high performance liquid chromatography (RP-HPLC)