色谱

色谱

• 特别策划 • 上一篇    

含氟甲喹残留的鳗鲡肉糜自然基体标准样品的制备

余孔捷*, 杨方, 刘正才, 黄杰, 李耀平, 陈枝华   

  1. 福建出入境检验检疫局, 国家鳗鱼与食源性微生物检测重点实验室, 福建省检验检疫技术研究重点实验室, 福建 福州 350001
  • 收稿日期:2011-04-14 修回日期:2011-05-29 出版日期:2011-07-28 发布日期:2011-08-19
  • 通讯作者: 余孔捷,研究员. Tel: (0591)87065545,
  • 基金资助:

    福建省科技重点项目(2007Y0001)和福建检验检疫局科技计划项目(FK2010-23)

Preparation of natural matrix standard sample of minced eel for quality control of flumequine residue

YU Kongjie*, YANG Fang, LIU Zhengcai, HUANG Jie, LI Yaoping, CHEN Zhihua   

  1. Fujian Entry-Exit Inspection and Quarantine Bureau, State Key Testing Laboratory of Eel and Food Origin Microorganism, Fujian Key Laboratory of Inspection and Quarantine Technology Research, Fuzhou 350001, China
  • Received:2011-04-14 Revised:2011-05-29 Online:2011-07-28 Published:2011-08-19

PDF

374

被引次数

13

摘要： 为了获得与实际检测样品完全一致的检测质量控制样品,研制了含氟甲喹的自然基体鳗鲡肉糜标准样品。在模拟人工养殖的自然条件下适量施用氟甲喹药物,采集符合预期水平(氟甲喹含量为5～10 μg/kg)的阳性材料。取带皮的阳性肌肉均质,过10目筛网。样品用尼龙/聚丙烯(PA/CPP)耐蒸煮复合膜袋包装,经Co60-γ射线12 kGy剂量的辐照灭菌;经均匀性检验、稳定性检验、中国合格评定国家认可委员会认可实验室液相色谱-串联质谱法检测协同定值。所建立的制备方法为含药物残留的鳗鲡肉糜的自然基体标准样品的实验室制备提供了一种选择。

关键词: 氟甲喹残留, 鳗鲡肉糜, 制备, 自然基体标准样品

Abstract: In order to obtain quality control samples for the analysis of flumequine residue in eel which are consistent with real detection samples, minced eel was prepared as natural matrix standard samples containing flumequine. The results showed that through the administration of drug application and appropriate time of sample acquisition, the natural positive matrix containing the expected levels (5~10 μg/kg) of flumequine and the representative of actual samples at the original state could be obtained. The uniformity of the material was ensured by homogenizing and paddle type blending. The irradiation of Co60-γ rays at 12 kGy and the packaging material could prevent the degradation of the minced samples at ambient temperature and facilitate the inter-laboratory transfer in any season.

Key words: minced eel, natural matrix standard, preparation, flumequine residue