色谱

色谱

• 研究论文 • 上一篇    下一篇

闪蒸-气相色谱指纹图谱及系统聚类分析用于烟用香精香料的测定

蒋健1, 杨君1, 黄芳芳1, 许式强1, 王晓晴2, 郑晓2, 潘再法2, 王丽丽2*   

  1. 1. 浙江中烟工业有限责任公司技术中心, 浙江 杭州 310008; 2. 浙江工业大学化学工程与材料学院, 浙江 杭州 310014
  • 收稿日期:2011-01-26 修回日期:2011-04-26 出版日期:2011-06-28 发布日期:2011-07-25
  • 通讯作者: 王丽丽,博士,教授,主要从事色谱分析工作. Tel: (0571)88320416,

Flash evaporation-gas chromatographic fingerprints with hierarchical cluster analysis for the determination of cigarette flavors

JIANG Jian1, YANG Jun1, HUANG Fangfang1, XU Shiqiang1, WANG Xiaoqing2, ZHENG Xiao2, PAN Zaifa2, WANG Lili2*   

  1. 1. Technology Center, China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou 310008, China; 2. College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, China
  • Received:2011-01-26 Revised:2011-04-26 Online:2011-06-28 Published:2011-07-25

PDF

556

被引次数

22

摘要： 采用闪蒸-气相色谱法(FE-GC)对不同黏度的烟用香精香料样品进行测定,考察了影响测定的主要因素。结果表明,0.40 mg样品在350 ℃下进行FE-GC分析,可以得到重现性良好的色谱图。与超声辅助液-液萃取-气相色谱法(ULLE-GC)分析结果的比较表明,FE-GC适用于不同黏度样品的分析。采用该法检测了中等黏度的烟用香精香料1184号8个批次样品,并建立其指纹图谱。利用系统聚类分析法可以明显区分1184号样品及掺兑10%～30%其他种类烟用香精香料的样品。FE-GC法简便、快速、灵敏,适用于不同黏度的烟用香精香料样品的检测和质量控制分析。

关键词: 闪蒸-气相色谱法, 系统聚类分析, 烟用香精香料, 指纹图谱

Abstract: A method of flash evaporation-gas chromatographic (FE-GC) fingerprints with hierarchical cluster analysis was established for the quality control of the cigarette flavor samples. An amount of 0.40 mg of the cigarette flavor sample was analyzed by FE-GC at optimized the flash evaporation temperature, 350 ℃. Good reproducibility for chromatograms was obtained with the relative standard deviations (RSDs) of the retention time less than 0.22%, and the relative peak area less than 9.37%. Compared with ultrasound-assisted liquid-liquid extraction-gas chromatography (ULLE-GC), FE-GC was proved to be more suitable for the analysis of cigarette flavor samples with various viscosity. The similarity correlation coefficients were above 0.998 for the 8 batches of cigarette flavor No.1184. Furthermore, these samples could be successfully distinguished from the ones mixed with other kinds of flavor components at the mixed levels of 10%~30% by hierarchical cluster analysis. It demonstrated that the method of FE-GC fingerprints with hierarchical cluster analysis is simple, rapid, accurate, and suitable for the quality control of cigarette flavors.

Key words: cigarette flavors, fingerprint chromatogram, hierarchical cluster analysis, flash evaporation-gas chromatography (FE-GC)