色谱

色谱 ›› 2012, Vol. 30 ›› Issue (04): 400-403.

• 特别策划 • 上一篇    下一篇

毛细管离子色谱法测定酒和饮料中阳离子

张婷婷*, 叶明立, 胡忠阳, 潘广文, 梁立娜, 吴述超   

  1. 赛默飞世尔科技有限公司上海实验室, 上海 201203
  • 收稿日期:2011-12-19 修回日期:2012-02-23 出版日期:2012-04-28 发布日期:2012-04-28
  • 通讯作者: 张婷婷,硕士,主要研究方向为离子色谱分离应用.
  • 基金资助:

    安徽省教育厅高校省级科学研究项目(KJ2011B211)

Determination of cations in wines and beverages based on capillary ion chromatography

ZHANG Tingting*, YE Mingli, HU Zhongyang, PAN Guangwen, LIANG Lina, WU Shuchao   

  1. Shanghai Lab., Thermo Fisher Scientific Inc., Shanghai 201203, China
  • Received:2011-12-19 Revised:2012-02-23 Online:2012-04-28 Published:2012-04-28

PDF

251

被引次数

10

摘要： 建立了毛细管离子色谱测定酒、饮料等样品中阳离子的分析方法。使用毛细管离子色谱柱IonPac CS12A(250 mm×0.4 mm, 8 μm),以甲基磺酸淋洗液发生器(EGC-MSA)产生18 mmol/L的甲基磺酸为流动相,进样量0.4 μL,在流速0.01 mL/min的条件下,采用自循环抑制电导检测的方法对啤酒、葡萄酒、白酒、果汁及奶茶等样品中的阳离子含量进行检测。结果表明,毛细管离子色谱法能满足阳离子含量的测定要求,系统稳定不易堵,在灵敏度方面优于常规离子色谱系统。该方法能够快速、准确地测定酒、饮料等样品中的5种阳离子(钠、铵、钾、镁和钙),回归方程的相关系数在0.9997以上,实际样品的加标回收率为95.2%～103.3%。该方法具有灵敏度高,操作简单,环境友好的特点。

关键词: 酒, 毛细管离子色谱, 阳离子, 抑制电导检测, 饮料

Abstract: A new method for the determination of five cations (sodium, ammonium, potassium, magnesium and calcium) in wines and beverages was developed and validated based on capillary ion chromatography. With a capillary ion exchange column (IonPac CS12A, 250 mm×0.4 mm, 8 μm) and 18 mmol/L methanesulfonic acid (MSA) elution, the five cations can be well separated in 15 min. After suppression with a capillary suppressor (CCES 300), the background was much decreased, and the sensitivities of the cations were greatly improved. For the milky tea, acetonitrile was added into the sample solution to deposit the proteins. With the pretreatment of an OnGuard RP cartridge to remove hydrophobic substances in the sample, the developed method can be applied to the determination of the cations in wines and beverages. The calibration curves of peak area versus concentration gave correlation coefficients more than 0.9997 for these cations. Average recoveries were between 95.2%~103.3%. The method is suitable for the determination of alkali metals and alkaline-earth metals in wines and beverages. The capillary ion chromatography provides analysis with less solvent consumption and better column efficiency, also possesses the advantages of high sensitivity, good selectivity and environmental friendly.

Key words: beverages, suppressor conductivity detection, wines, capillary ion chromatography (CIC), cations