色谱

色谱 ›› 2012, Vol. 30 ›› Issue (10): 1081-1088.DOI: 10.3724/SP.J.1123.2012.05030

• 研究论文 • 上一篇    下一篇

Thin layer chromatographic separation of cobalt from nickel on impregnated silica gel layers: quantitative determination by digital image analysis

P A MOHAMED NAJAR*, R G SONALI, M T NIMJE, K V RAMANA RAO   

  1. Jawaharlal Nehru Aluminium Research Development and Design Centre, Nagpur 440 023, India
  • 收稿日期:2012-05-17 修回日期:2012-06-28 出版日期:2012-10-28 发布日期:2012-10-17
  • 通讯作者: P A MOHAMED NAJAR. Fax: +91-7104-220942, E-mail: najarp@hotmail.com.
  • 基金资助:

    Science & Technology Wing, Ministry of Mines, Government of India is acknowledged for financial support to the project on “Development of Rapid Analytical Procedures for Cobalt, Chromium and Nickel”.

Thin layer chromatographic separation of cobalt from nickel on impregnated silica gel layers: quantitative determination by digital image analysis

P A MOHAMED NAJAR*, R G SONALI, M T NIMJE, K V RAMANA RAO   

  1. Jawaharlal Nehru Aluminium Research Development and Design Centre, Nagpur 440 023, India
  • Received:2012-05-17 Revised:2012-06-28 Online:2012-10-28 Published:2012-10-17
  • Contact: P A MOHAMED NAJAR. Fax: +91-7104-220942, E-mail: najarp@hotmail.com.

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摘要： Thin layer chromatography (TLC) of cobalt and nickel has been performed on silica gel layers induced with alkali mediated cellulose extract. A novel combination of 10% aqueous solutions of Tween-20 and potassium thiocyanate in 1∶1 (v/v) was identified as the best mobile phase for the selective separation of Co2+from Ni2+on the impregnated Silica Gel G layers. The chromatographic characteristics of the cations were studied and the limits of detection as well as the limits of quantification for Co2+and Ni2+were determined. The quantitative estimation of the cations was achieved from the digital image analysis of respective chromatograms. The proposed quantitative method was successfully applied with 0-0.50% error for the determination of Co2+from Ni2+in spiked samples of bauxite, soil and rock containing common cations such as Al3+, Fe2+, Ti4+, Zn2+, Mn2+, Cu2+, Cr6+, Mg2+, etc. under the optimized chromatographic conditions.

关键词: cobalt, digital image analysis, nickel, quantification, thin layer chromatography (TLC)

Abstract: Thin layer chromatography (TLC) of cobalt and nickel has been performed on silica gel layers induced with alkali mediated cellulose extract. A novel combination of 10% aqueous solutions of Tween-20 and potassium thiocyanate in 1∶1 (v/v) was identified as the best mobile phase for the selective separation of Co2+from Ni2+on the impregnated Silica Gel G layers. The chromatographic characteristics of the cations were studied and the limits of detection as well as the limits of quantification for Co2+and Ni2+were determined. The quantitative estimation of the cations was achieved from the digital image analysis of respective chromatograms. The proposed quantitative method was successfully applied with 0-0.50% error for the determination of Co2+from Ni2+in spiked samples of bauxite, soil and rock containing common cations such as Al3+, Fe2+, Ti4+, Zn2+, Mn2+, Cu2+, Cr6+, Mg2+, etc. under the optimized chromatographic conditions.

Key words: cobalt, digital image analysis, nickel, quantification, thin layer chromatography (TLC)

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