色谱

色谱 ›› 2013, Vol. 31 ›› Issue (5): 490-493.DOI: 10.3724/SP.J.1123.2012.12012

• 技术与应用 • 上一篇    

固相萃取-高效液相色谱-可变波长检测法同时测定调味品中甜味剂和人工合成色素

赵飞, 高广慧*, 那海秋, 马殿君, 王晓黎   

  1. 辽宁省食品药品检验所, 辽宁 沈阳 110023
  • 收稿日期:2012-12-05 修回日期:2013-01-25 出版日期:2013-05-28 发布日期:2013-05-16
  • 通讯作者: 高广慧,Tel:(024)25428020,E-mail:gghggh2000@163.com.

Simultaneous determination of saccharin sodium and synthetic colours in flavourings by solid phase extraction and high performance liquid chromatography

ZHAO Fei, GAO Guanghui*, NA Haiqiu, MA Dianjun, WANG Xiaoli   

  1. Liaoning Institute for Food and Drug Control, Shenyang 110023, China
  • Received:2012-12-05 Revised:2013-01-25 Online:2013-05-28 Published:2013-05-16

PDF

227

被引次数

27

摘要：

建立了同时测定调味品中糖精钠和合成色素的固相萃取-高效液相色谱(SPE-HPLC)分析方法。样品经石油醚低温去除油脂,聚酰胺固相萃取柱净化和富集,然后进行HPLC分析。采用反相C18色谱柱,以甲醇和0.02 mol/L醋酸铵为流动相进行梯度洗脱,使用可变波长分别在230、510、484和510 nm进行检测。实验结果表明:糖精钠与合成色素的分离效果良好,回收率为88.6%～97.1%,相对标准偏差小于5%;方法检出限均可达到0.05 mg/kg。该方法操作简单,结果准确可靠,重现性好,适用于大批量调味品中甜味剂和人工合成色素的同时检测。

关键词: 调味品, 高效液相色谱法, 固相萃取, 合成色素, 糖精钠

Abstract:

A method for the simultaneous determination of saccharin sodium and synthetic colours in flavourings by solid phase extraction (SPE) and high performance liquid chromatography with variable wavelengths was established. After degreased with petroleum ether, extracted, purified with SPE, and concentrated, the sample was separated on a C18 column with a gradient elution of 0.02 mol/L ammonium acetate and methanol, and detected at 230, 510, 484 and 510 nm, separately. The saccharin sodium and synthetic colours were separated with this method. The recoveries were 88.6%-97.1% with the RSD<5%. The limit of detection was 0.05 mg/kg for all the analytes. The proposed method is simple, reliable and reproducible, and especially suitable for a lot of sample detections of saccharin sodium and synthetic colours in flavourings.

Key words: flavourings, high performance liquid chromatography (HPLC), saccharin sodium, solid phase extraction (SPE), synthetic colours

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