色谱

色谱 ›› 2013, Vol. 31 ›› Issue (6): 567-571.DOI: 10.3724/SP.J.1123.2012.12052

• 研究论文 • 上一篇    下一篇

高效液相色谱法同时测定水体中的环丙沙星和氟甲喹

李璐, 刘菲, 陈鸿汉, 秦晓鹏   

  1. 水资源与环境工程北京市重点实验室, 中国地质大学(北京), 北京 100083
  • 收稿日期:2012-12-28 修回日期:2013-01-31 出版日期:2013-06-28 发布日期:2013-06-06
  • 通讯作者: 刘菲
  • 基金资助:

    国家自然科学基金项目(51078338).

Simultaneous determination of ciprofloxacin and flumequine in water samples by high performance liquid chromatography

LI Lu, LIU Fei, CHEN Honghan, QIN Xiaopeng   

  1. Beijing Key Laboratory of Water Resource & Environmental Engineering, China University of Geosciences (Beijing), Beijing 100083, China
  • Received:2012-12-28 Revised:2013-01-31 Online:2013-06-28 Published:2013-06-06
  • Contact: 10.3724/SP.J.1123.2012.12052

PDF

194

被引次数

10

摘要：

为了满足实验室测试和野外测试需要,也为其他学者研究不同抗生素之间的相互作用提供相关的资料,本研究选用环丙沙星(CIP)和氟甲喹(FLU)作为代表,建立了一种简单、稳定、易普及的高效液相色谱法用于同时快速测定水体中两种氟喹诺酮类抗生素的含量,并讨论了不同流动相及其比例和水样中几种常见阴、阳离子(Ca2+、Mg2+、Fe3+、Al3+、SO42-和HCO3-)对抗生素测定的影响。结果表明:三乙胺对改善柱效有明显效果;低浓度离子对测试影响不大,但Fe3+和Al3+可能与固定相的表面羟基或测试组分发生配合作用,造成基线不稳。实验结果对其他研究者对于流动相的选择与优化具有借鉴意义。

关键词: 氟甲喹, 氟喹诺酮, 高效液相色谱, 环丙沙星, 水体

Abstract:

In order to meet the field sample measurements and laboratory research, a simple, stable, and popular high performance liquid chromatographic (HPLC) method was established for the determination of the concentrations of two fluoroquinolone antibiotics in water samples simultaneously and rapidly. Thus, it could provide valuable information for other scholars to study the interactions between different antibiotics. Ciprofloxacin (CIP) and flumequine (FLU) were taken as the target contaminants because of their wide application in medical career. Furthermore, this study also investigated the different types of mobile phase, different mobile phase ratios, common ions (Ca2+, Mg2+, Fe3+, Al3+, SO42-and HCO3-) in water samples and other factors on CIP and FLU measurements with this method. The results showed that triethylamine has obvious effect on improving column efficiency. Low concentrations of ions had little effect on the test, but Fe3+and Al3+might cause baseline instability, because Fe3+and Al3+may form complexes with the surface hydroxyl groups of the stationary phase or the test components. The results may be referred by other workers for the optimization of the determination conditions.

Key words: ciprofloxacin, flumequine, fluoroquinolones, high performance liquid chromatography (HPLC), water samples

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