色谱

色谱 ›› 2013, Vol. 31 ›› Issue (6): 510-513.DOI: 10.3724/SP.J.1123.2013.01003

• 特别策划：定量蛋白质组学专栏 • 上一篇    下一篇

利用液相色谱-质谱多反应监测方法测定食物中主要的牛奶过敏原α-酪蛋白

冯小燕1, 张津2, 吕美玲2, 高明霞1, 张祥民1   

  1. 1. 复旦大学化学系, 上海 200433;
    2. 安捷伦科技(中国)有限公司, 北京 100102
  • 收稿日期:2013-01-05 修回日期:2013-02-02 出版日期:2013-06-28 发布日期:2013-06-06
  • 通讯作者: 张祥民
  • 基金资助:

    科技部基础研究计划项目(2012CB910604);国家自然科学基金项目(21275034,21027002,21175026).

Detection of the major milk allergenα-casein in food with liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode

FENG Xiaoyan1, ZHANG Jin2, LV Meiling2, GAO Mingxia1, ZHANG Xiangmin1   

  1. 1. Department of Chemistry, Fudan University, Shanghai 200433, China;
    2. Agilent Technologies (China) Co., Ltd., Beijing 100102, China
  • Received:2013-01-05 Revised:2013-02-02 Online:2013-06-28 Published:2013-06-06
  • Contact: 10.3724/SP.J.1123.2013.01003

PDF

389

摘要：

采用液相色谱-质谱串联的多反应监测技术检测食物中的牛奶过敏原成分α-酪蛋白,检出限达到了0.5 mg/L,与目前已报道的检出限水平相当。该过敏原成分在0.5~250 mg/L范围内线性关系良好,说明本方法对于食品中牛奶过敏原成分的检测具有极好的实际应用价值。

关键词: α-酪蛋白, 多反应监测, 牛奶过敏原, 食品, 液相色谱-质谱

Abstract:

A new method of detecting milk allergen protein in food was established. Using multiple reaction monitoring (MRM) mode in high performance liquid chromatography-mass spectrometry (HPLC-MS) to detect milk allergen protein α-casein is successful and convenient. All transitions gave a linear response in the chosen concentration range of the milk allergen protein (between 0.5 and 250 mg/L total soluble protein, 5 data points), and the detection limit is 0.5 mg/L of α-casein. Food samples from markets were tested. The results showed that MRM method could be used to detect the milk allergen in food products.

Key words: α-casein, food, liquid chromatography-mass spectrometry(LC-MS), milk allergen, multiple reaction monitoring (MRM)

中图分类号: