色谱

色谱 ›› 2013, Vol. 31 ›› Issue (6): 518-521.DOI: 10.3724/SP.J.1123.2013.01022

• 特别策划：定量蛋白质组学专栏 • 上一篇    下一篇

一种基于硝酸纤维素膜的新颖便捷的芯片对循环肿瘤细胞的高效捕获

张鹏1, 孙长龙1, 张人1, 高明霞1,2, 张祥民1,2   

  1. 1. 复旦大学化学系, 上海 200433;
    2. 复旦大学生物医学研究院, 上海 200433
  • 收稿日期:2013-01-10 修回日期:2013-02-25 出版日期:2013-06-28 发布日期:2013-06-06
  • 通讯作者: 高明霞, 张祥民
  • 基金资助:

    科技部基础研究计划项目(2012CB910604);国家自然科学基金项目(21275034,21027002,21175026).

A novel and facile microchip based on nitrocellulose membrane toward efficient capture of circulating tumor cells

ZHANG Peng1, SUN Changlong1, ZHANG Ren1, GAO Mingxia1,2, ZHANG Xiangmin1,2   

  1. 1. Department of Chemistry, Fudan University, Shanghai 200433, China;
    2. Institute of Biomedical Sciences, Fudan University, Shanghai 200433, China
  • Received:2013-01-10 Revised:2013-02-25 Online:2013-06-28 Published:2013-06-06
  • Contact: 10.3724/SP.J.1123.2013.01022

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摘要：

开发了一种新颖便捷的循环肿瘤细胞(CTC)捕获芯片用于癌细胞的分离和检测。CTC芯片以硝酸纤维素膜为基底制备,利用其对蛋白质的超强吸附能力来结合抗体,简便高效,便于将来大规模的推广应用。以非小肺癌细胞NCI-H1650为目标靶细胞,证明了CTC芯片对癌细胞具有很高的捕获效能。向1 mL正常人血液中加入500个癌细胞模拟病人血样的分析中成功检测到了182个癌细胞,预示了CTC芯片将来在临床应用上的巨大潜力。

关键词: 抗体, 硝酸纤维素膜, 芯片, 循环肿瘤细胞

Abstract:

A novel and facile circulating tumor cell (CTC) microchip has been developed for the isolation and detection of cancer cells. The CTC microchip was prepared based on the nitrocellulose membrane substrate, which shows high affinity to proteins and hence can adsorb antibodies naturally. We employed non-small-cells of lung cancer NCI-H1650 as target cells and testified the high capture efficacy of the CTC microchip. Furthermore, we spiked 500 cancer cells to 1 mL healthy donor’s whole blood in order to simulate the detection of CTC in patient and detected 182 cancer cells ultimately, indicating the huge application potential in the future.

Key words: antibody, circulating tumor cells (CTCs), microchip, nitrocellulose membrane (NCM)

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