色谱

色谱 ›› 2013, Vol. 31 ›› Issue (9): 821-824.DOI: 10.3724/SP.J.1123.2013.02003

• 研究论文 • 上一篇    下一篇

万古霉素键合固定相的分离性能

黄兰淇1, 周魏华1, 陈义旺1, 张凌怡2, 万益群1, 张维冰1,2   

  1. 1. 南昌大学化学系, 江西 南昌 330031;
    2. 华东理工大学化学与分子工程学院, 上海 200237
  • 收稿日期:2013-02-04 修回日期:2013-03-03 出版日期:2013-09-28 发布日期:2013-09-02
  • 通讯作者: 周魏华
  • 基金资助:

    食品科学与技术国家重点实验室2010年度开放基金(SKLF-KF-201002).

Separation performance of vancomycin-bonded stationary phase

HUANG Lanqi1, ZHOU Weihua1, CHEN Yiwang1, ZHANG Lingyi2, WAN Yiqun1, ZHANG Weibing1,2   

  1. 1. Department of Chemistry, Nanchang University, Nanchang 330031, China;
    2. School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2013-02-04 Revised:2013-03-03 Online:2013-09-28 Published:2013-09-02

PDF

350

被引次数

6

摘要：

万古霉素作为一种大环抗生素,具有复杂的分子结构。在充分考虑万古霉素分子结构特征的情况下,采用戊二醛间隔臂法制备了万古霉素键合固定相,在反相、亲水、离子交换等分离模式下研究了其色谱分离性能。结果表明,当流动相中有机调节剂含量较低时,该色谱柱表现出典型的反相色谱分离模式特征;随着有机调节剂含量的增加,逐渐转变成亲水模式,分离特性发生明显改变。由于万古霉素分子结构中含有可以解离的氨基,因此该固定相也能够用于阴离子交换模式下的分析方法的发展。分别在反相、亲水和阴离子交换模式下,将其应用于扑尔敏等多种非对映体药物和新型甜味剂甜菊糖的高效液相色谱分离;仅通过改变分离条件,即可在3种不同分离模式下完成分离。这些结果可以为新型色谱固定相的设计,以及发展采用特殊结构改性基团的色谱固定相在相应分离模式下的分析方法提供指导。

关键词: 分离模式, 固定相, 万古霉素

Abstract:

Vancomycin is one of the macrocyclic antibiotics with complex molecular structure. Based on the various functional groups of vancomycin, a vancomycin-bonded stationary phase using glutaraldehyde as the spacer was prepared. Its chromatographic properties in reversed-phase, ion exchange and hydrophilic phase modes were investigated separately. The prepared stationary phase showed the typical characteristic of the reversed-phase stationary, when the organic solvent content was low in the mobile phase. On the contrary, its chromatographic characteristic transformed into hydrophilic phase mode with the organic solvent content increased in the mobile phase. Owing to the amino groups of vancomycin, ion exchange mode can also be applied to the separation method development. The vancomycin-bonded stationary phase was applied to the separation of eight achiral drugs and stevioside in reversed-phase, ion exchange and hydrophilic phase modes. The separations were achieved in three different kinds of separation modes by using appropriate chromatographic conditions. The results provide guidance for the design of new types of stationary phase, and method development of chromatographic stationary phases modified by special compounds with complex construction in corresponding separation modes.

Key words: separation mode, stationary phase, vancomycin

中图分类号: