色谱

色谱 ›› 2013, Vol. 31 ›› Issue (9): 875-877.DOI: 10.3724/SP.J.1123.2013.03014

• 研究论文 • 上一篇    下一篇

高效液相色谱法测定乳制品中的双氰胺

陈小珍, 陈万勤, 王瑾, 黄丽英, 张东雷   

  1. 浙江省质量检测科学研究院, 浙江省食品安全重点实验室, 浙江 杭州 310013
  • 收稿日期:2013-03-05 修回日期:2013-04-22 出版日期:2013-09-28 发布日期:2013-09-02
  • 通讯作者: 陈小珍
  • 基金资助:

    浙江省质量技术监督系统科研计划项目(20120205).

Determination of dicyandiamide in dairy products by high performance liquid chromatography

CHEN Xiaozhen, CHEN Wanqin, WANG Jin, HUANG Liying, ZHANG Donglei   

  1. Zhejiang Institute of Quality Inspection Science, Key Laboratory for Food Safety of Zhejiang Province, Hangzhou 310013, China
  • Received:2013-03-05 Revised:2013-04-22 Online:2013-09-28 Published:2013-09-02

PDF

421

被引次数

19

摘要：

建立了乳制品中双氰胺的高效液相色谱(HPLC)检测方法。样品经乙腈提取后采用HPLC测定。优化后的色谱条件:XBridge Amide柱(250 mm×4.6 mm, 3.5 μm),流动相为乙腈和水(体积比为90:10,含0.2%甲酸),流速为1.0 mL/min,检测波长为218 nm。方法在0.5~50 mg/L范围内线性关系良好,相关系数(r2)为0.9999,回收率在96.7%~101.0%之间,RSD为4.5%~4.9%(n=6),检出限为0.2 mg/kg(S/N=3),定量限为0.5 mg/kg(S/N=10)。该方法简单、准确、灵敏度高,适用于各种乳制品中双氰胺的检测。

关键词: 高效液相色谱法, 乳制品, 双氰胺

Abstract:

A high performance liquid chromatographic (HPLC) method for the determination of dicyandiamide in dairy products was developed. The sample was extracted by acetonitrile, separated on an XBridge Amide column (250 mm×4.6 mm, 3.5 μm) using acetonitrile-water (90/10, v/v) containing 0.2%(v/v) formic acid as the mobile phase at a flow rate of 1.0 mL/min, and determined by a photodiode array detector at the wavelength of 218 nm. The calibration curve was linear in the range of 0.5-50 mg/L with the correlation coefficient (r2) of 0.9999. The recoveries were from 96.7% to 101.0% with the relative standard deviations (RSDs) in the range of 4.5% to 4.9%(n=6). The limit of detection was 0.2 mg/kg, and the limit of quantification was 0.5 mg/kg. The method is simple, sensitive, accurate and precise for the determination of dicyandiamide in dairy products.

Key words: dairy products, dicyandiamide, high performance liquid chromatography (HPLC)

中图分类号: